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DMGDH Polyclonal antibody, PBS Only

DMGDH Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 24813-1-PBS

Print datasheet

Synonyms

ME2GLYDH, M2GD, EC:1.5.8.4, DMGDHD, dimethylglycine dehydrogenase

验证数据展示

  • WB analysis using 24813-1-AP (same clone as 24813-1-PBS)

    WB analysis using 24813-1-AP (same clone as 24813-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24813-1-AP (DMGDH antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 24813-1-AP (same clone as 24813-1-PBS)

    IF Staining of HepG2 using 24813-1-AP (same clone as 24813-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using DMGDH antibody (24813-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 24813-1-AP (same clone as 24813-1-PBS)

    FC experiment of HepG2 using 24813-1-AP (same clone as 24813-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug DMGDH Polyclonal antibody (24813-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 24813-1-AP (same clone as 24813-1-PBS)

    IHC staining of human liver cancer using 24813-1-AP (same clone as 24813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24813-1-AP (DMGDH Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 24813-1-AP (same clone as 24813-1-PBS)

    IHC staining of human liver cancer using 24813-1-AP (same clone as 24813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24813-1-AP (DMGDH Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

产品信息

24813-1-PBS targets DMGDH in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DMGDH fusion protein Ag20529 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dimethylglycine dehydrogenase
Synonyms ME2GLYDH, M2GD, EC:1.5.8.4, DMGDHD, dimethylglycine dehydrogenase
Calculated Molecular Weight 866 aa, 97 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC022388
Gene Symbol DMGDH
Gene ID (NCBI) 29958
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UI17
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DMGDH(Dimethylglycine dehydrogenase, mitochondrial) is also named as ME2GLYDH and belongs to the GcvT family. It catalyzes the oxidative demethylation of dimethylglycine to form sarcosine and it plays a role in choline catabolism in the liver. The full length protein has a transit peptide.