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DMKN Polyclonal antibody

DMKN Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16252-1-AP

Print datasheet

Synonyms

dermokine, DMKN, UNQ729, ZD52F10

验证数据展示

  • WB analysis of mouse ovary using 16252-1-AP

    WB analysis of mouse ovary using 16252-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16252-1-AP (DMKN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 16252-1-AP

    IP experiment of mouse lung using 16252-1-AP

    IP result of anti-DMKN (IP:16252-1-AP, 4ug; Detection:16252-1-AP 1:500) with mouse lung tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse ovary tissue, mouse lung tissue
Positive IP detected inmouse lung tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16252-1-AP targets DMKN in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen DMKN fusion protein Ag9242 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dermokine
Synonyms dermokine, DMKN, UNQ729, ZD52F10
Calculated Molecular Weight 476 aa, 47 kDa
Observed Molecular Weight 48-55 kDa
GenBank Accession NumberBC011886
Gene Symbol DMKN
Gene ID (NCBI) 93099
RRIDAB_10596473
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6E0U4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DMKN, also named as epidermis-specific secreted protein SK30/SK89, acts as a soluble regulator of keratinocyte differentiation. DMKN has 16 isoforms with MW 9-20 kDa, 35-38 kDa and 42-48 kDa.

实验方案

Product Specific Protocols
WB protocol for DMKN antibody 16252-1-APDownload protocol
IP protocol for DMKN antibody 16252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell Proteomics

Deletion of cysteine cathepsins B or L yields differential impacts on murine skin proteome and degradome.

Authors - Tholen Stefan S
View Article

Mol Cell Proteomics

Matrix metalloproteinase 10 degradomics in keratinocytes and epidermal tissue identifies bioactive substrates with pleiotropic functions.

Authors - Pascal Schlage
View Article