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DMRT1 Polyclonal antibody

DMRT1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 14313-1-AP

Print datasheet

Synonyms

Doublesex- and mab-3-related transcription factor 1, DMT1, CT154

验证数据展示

  • WB analysis using 14313-1-AP

    WB analysis using 14313-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14313-1-AP (DMRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 14313-1-AP

    IHC staining of mouse testis using 14313-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14313-1-AP (DMRT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DMRT1 antibody included
  • IP experiment of mouse testis using 14313-1-AP

    IP experiment of mouse testis using 14313-1-AP

    IP result of anti-DMRT1 (IP:14313-1-AP, 4ug; Detection:14313-1-AP 1:500) with mouse testis tissue lysate 2400 ug.

  • IF Staining of mouse testis using 14313-1-AP

    IF Staining of mouse testis using 14313-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DMRT1 antibody (14313-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 14313-1-AP

    IF Staining of mouse testis using 14313-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DMRT1 antibody (14313-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue, human testis
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Planning an IHC experiment? We recommend our IHCeasy DMRT1 Ready-To-Use IHC Kit. DMRT1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14313-1-AP targets DMRT1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DMRT1 fusion protein Ag5547 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name doublesex and mab-3 related transcription factor 1
Synonyms Doublesex- and mab-3-related transcription factor 1, DMT1, CT154
Calculated Molecular Weight 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC040847
Gene Symbol DMRT1
Gene ID (NCBI) 1761
RRIDAB_2918035
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5R6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DMRT1, also named DMT1, belongs to the DMRT family. DMRT1 is one of the most conserved transcription factors in a variety of species and is involved in sex determination and germ cell differentiation. DMRT1 prevents the sexual switch from male to female, represses pluripotency, and balances mitosis versus meiosis. DMRT1 also maintains spermatogenesis and replenishes mGSCs by collaborating with Plzf and Sal-like protein 4 (PMID: 33420764). DMRT1 has 3 isoforms with the molecular mass of 18, 29, and 39 kDa.

实验方案

Product Specific Protocols
WB protocol for DMRT1 antibody 14313-1-APDownload protocol
IHC protocol for DMRT1 antibody 14313-1-APDownload protocol
IF protocol for DMRT1 antibody 14313-1-APDownload protocol
IP protocol for DMRT1 antibody 14313-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols