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DMRT1 Polyclonal antibody

DMRT1 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 28149-1-AP

Print datasheet

Synonyms

DMRT1, DMT1

验证数据展示

  • WB analysis using 28149-1-AP

    WB analysis using 28149-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28149-1-AP (DMRT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 28149-1-AP

    IF Staining of mouse testis using 28149-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DMRT1 antibody (28149-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, DU 145 cells, LNCaP cells, PC-3 cells, SKOV-3 cells
Positive IF-P detected inmouse testis tissue
Planning an IHC experiment? We recommend our IHCeasy DMRT1 Ready-To-Use IHC Kit. DMRT1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28149-1-AP targets DMRT1 in WB, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen DMRT1 fusion protein Ag28006 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name doublesex and mab-3 related transcription factor 1
Synonyms DMRT1, DMT1
Calculated Molecular Weight 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC040847
Gene Symbol DMRT1
Gene ID (NCBI) 1761
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5R6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DMRT1, also named DMT1, belongs to the DMRT family. DMRT1 is one of the most conserved transcription factors in a variety of species and is involved in sex determination and germ cell differentiation. DMRT1 prevents the sexual switch from male to female, represses pluripotency, and balances mitosis versus meiosis. DMRT1 also maintains spermatogenesis and replenishes mGSCs by collaborating with Plzf and Sal-like protein 4 (PMID: 33420764). DMRT1 has 3 isoforms with the molecular mass of 18, 29, and 39 kDa.

实验方案

Product Specific Protocols
WB protocol for DMRT1 antibody 28149-1-APDownload protocol
IF protocol for DMRT1 antibody 28149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols