验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, mouse thymus tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18727-1-AP targets DNA2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DNA replication helicase 2 homolog (yeast) |
| 别名 | DNA2L, KIAA0083 |
| 计算分子量 | 120 kDa |
| 观测分子量 | 120-130 kDa |
| GenBank蛋白编号 | NM_001080449 |
| 基因名称 | DNA2 |
| Gene ID (NCBI) | 1763 |
| RRID | AB_10637998 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P51530 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNA2(DNA replication ATP-dependent helicase/nuclease DNA2) is also named as DNA2L, KIAA0083 and belongs to the DNA2/NAM7 helicase family. This protein includes 2 domains: DNA replication nuclease and DNA replication ATP-dependent helicase. It is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability and it can interact with DNA polymerase gamma and stimulating its activity. It can also process intermediate 5' flap structures occuring in DNA replication long-patch base excision repair in mitochondria. It has 4 isoforms produced by alternative splicing. This antibody is specific to DNA2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DNA2 antibody 18727-1-AP | Download protocol |
| IHC protocol for DNA2 antibody 18727-1-AP | Download protocol |
| IF protocol for DNA2 antibody 18727-1-AP | Download protocol |
| IP protocol for DNA2 antibody 18727-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Stalled replication fork protection limits cGAS-STING and P-body-dependent innate immune signalling.
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Mol Cell Dynamic control of RNA-DNA hybrid formation orchestrates DNA2 activation at stalled forks by RNAPII and DDX39A
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Genes Dev Abro1 maintains genome stability and limits replication stress by protecting replication fork stability.
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Commun Biol The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells
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Cell Cycle The checkpoint clamp protein Rad9 facilitates DNA-end resection and prevents alternative non-homologous end joining.
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Cells Increased Resection at DSBs in G2-Phase Is a Unique Phenotype Associated with DNA-PKcs Defects That Is Not Shared by Other Factors of c-NHEJ.
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