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验证数据展示

  • WB analysis using 30073-1-AP

    WB analysis using 30073-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 30073-1-AP (DNAH6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 30073-1-AP

    IHC staining of mouse testis using 30073-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30073-1-AP (DNAH6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 30073-1-AP

    IHC staining of mouse testis using 30073-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30073-1-AP (DNAH6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of hTERT-RPE1 using 30073-1-AP

    IF Staining of hTERT-RPE1 using 30073-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using DNAH6 antibody (30073-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • IF Staining of hTERT-RPE1 using 30073-1-AP

    IF Staining of hTERT-RPE1 using 30073-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DNAH6 antibody (30073-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

DNAH6 Polyclonal antibody

DNAH6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30073-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Ciliary dynein heavy chain 6, DKFZp667K087, DNAH6, Dnahc6, DNHL1, Dynein heavy chain 6, axonemal, FLJ23529, FLJ37357, HL 2, HL2, KIAA1697

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30073-1-AP targets DNAH6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 DNAH6 fusion protein Ag32364 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dynein, axonemal, heavy chain 6
别名 Ciliary dynein heavy chain 6, DKFZp667K087, DNAH6, Dnahc6, DNHL1, Dynein heavy chain 6, axonemal, FLJ23529, FLJ37357, HL 2, HL2, KIAA1697
计算分子量475 kDa
观测分子量 475 kDa
GenBank蛋白编号BC104884
基因名称 DNAH6
Gene ID (NCBI) 1768
RRIDAB_2935510
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9C0G6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAH6(Dynein heavy chain 6, axonemal) is also named as DNAHC6, DNHL1, HL2, KIAA1697 and belongs to the dynein heavy chain family.Dyneins are microtubule-associated motor protein complexes composed of several heavy, light, and intermediate chains(PMID:8812413). It is a force generating protein of respiratory cilia and produces force towards the minus ends of microtubules.This antibody is specific to DNAH6.

实验方案

Product Specific Protocols
WB protocol for DNAH6 antibody 30073-1-APDownload protocol
IHC protocol for DNAH6 antibody 30073-1-APDownload protocol
IF protocol for DNAH6 antibody 30073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols