DNAI2 Polyclonal antibody

DNAI2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17533-1-AP

Print datasheet

Synonyms

Dynein axonemal intermediate chain 2, CILD9



经过测试的应用

Positive WB detected inmouse ovary tissue, HeLa cells, mouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17533-1-AP targets DNAI2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen DNAI2 fusion protein Ag11585 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dynein, axonemal, intermediate chain 2
Synonyms Dynein axonemal intermediate chain 2, CILD9
Calculated Molecular Weight 593 aa, 67 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC039582
Gene Symbol DNAI2
Gene ID (NCBI) 64446
RRIDAB_2096670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZS0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DNAI2 antibody 17533-1-APDownload protocol
IHC protocol for DNAI2 antibody 17533-1-APDownload protocol
IF protocol for DNAI2 antibody 17533-1-APDownload protocol
IP protocol for DNAI2 antibody 17533-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF,IP

Elife

ZMYND10 functions in a chaperone relay during axonemal dynein assembly.

Authors - Girish R Mali
mouseIF

J Mol Cell Biol

Production of transgenic mice by random recombination of targeted genes in female germline stem cells.

Authors - Zhang Yong Y
ratWB,IF

Front Cell Dev Biol

PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia

Authors - Tingting Zhang
mouseWB

Development

CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility

Authors - Keisuke Shimada
mouseWB

Exp Anim

The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum.

Authors - Haruhiko Miyata
humanWB

Ann Hum Genet

DNAH17 is associated with asthenozoospermia and multiple morphological abnormalities of sperm flagella.

Authors - Yanwei Sha