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  • KD/KO Validated

DNAJA1 Polyclonal antibody

DNAJA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 11713-1-AP

Print datasheet

Synonyms

hDj-2, HDJ2, hDJ 2, DNAJ2, DjA1



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, human testis tissue, HepG2 cells, human liver tissue, Jurkat cells, mouse lung tissue, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DNAJA1 Ready-To-Use IHC Kit. DNAJA1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11713-1-AP targets DNAJA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen DNAJA1 fusion protein Ag2284 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily A, member 1
Synonyms hDj-2, HDJ2, hDJ 2, DNAJ2, DjA1
Calculated Molecular Weight 397 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC008182
Gene Symbol DNAJA1
Gene ID (NCBI) 3301
RRIDAB_10637266
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31689
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.

实验方案

Product Specific Protocols
WB protocol for DNAJA1 antibody 11713-1-APDownload protocol
IHC protocol for DNAJA1 antibody 11713-1-APDownload protocol
IF protocol for DNAJA1 antibody 11713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nature

A cytosolic surveillance mechanism activates the mitochondrial UPR

Authors - F X Reymond Sutandy
  • KD Validated
humanIF

Nat Cell Biol

Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition

Authors - Shan Lu
humanWB,IP

Nat Commun

Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.

Authors - Jiawei Zhang
humanWB

J Clin Invest

Cul4A-DDB1-mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine.

Authors - Yajuan Zhang
  • KD Validated
mouseWB

Mol Ther Nucleic Acids

Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction

Authors - Jie Pan
humanWB

Sci Rep

Co-chaperones DNAJA1 and DNAJB6 are critical for regulation of polyglutamine aggregation.

Authors - Claudio Rodríguez-González
  • KO Validated