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DNAJA1 Polyclonal antibody

DNAJA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11713-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (2)

hDj-2, HDJ2, hDJ 2, DNAJ2, DjA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, human testis tissue, HepG2 cells, human liver tissue, Jurkat cells, mouse lung tissue, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DNAJA1 Ready-To-Use IHC Kit. DNAJA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11713-1-AP targets DNAJA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 DNAJA1 fusion protein Ag2284 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily A, member 1
别名 hDj-2, HDJ2, hDJ 2, DNAJ2, DjA1
计算分子量 397 aa, 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC008182
基因名称 DNAJA1
Gene ID (NCBI) 3301
RRIDAB_10637266
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31689
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.

实验方案

Product Specific Protocols
WB protocol for DNAJA1 antibody 11713-1-APDownload protocol
IHC protocol for DNAJA1 antibody 11713-1-APDownload protocol
IF protocol for DNAJA1 antibody 11713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nature

A cytosolic surveillance mechanism activates the mitochondrial UPR

Authors - F X Reymond Sutandy
  • KD Validated
humanIF

Nat Cell Biol

Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition

Authors - Shan Lu
humanWB,IP

Nat Commun

Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.

Authors - Jiawei Zhang
humanWB

J Clin Invest

Cul4A-DDB1-mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine.

Authors - Yajuan Zhang
  • KD Validated
mouseWB

Mol Ther Nucleic Acids

Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction

Authors - Jie Pan
humanWB

Sci Rep

Co-chaperones DNAJA1 and DNAJB6 are critical for regulation of polyglutamine aggregation.

Authors - Claudio Rodríguez-González
  • KO Validated
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