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验证数据展示

  • WB analysis of Jurkat using 11088-1-AP

    WB analysis of Jurkat using 11088-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11088-1-AP (TID1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 11088-1-AP

    IHC staining of human lung cancer using 11088-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11088-1-AP (TID1 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of K-562 using 11088-1-AP

    IP experiment of K-562 using 11088-1-AP

    IP result of anti-TID1 (IP:11088-1-AP, 4ug; Detection:11088-1-AP 1:500) with K-562 cells lysate 3200ug.

TID1 Polyclonal antibody

TID1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11088-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

DnaJ protein Tid 1, DNAJA3, HCA57, hTid 1, TID 1, TID1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11088-1-AP targets TID1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 TID1 fusion protein Ag1558 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily A, member 3
别名 DnaJ protein Tid 1, DNAJA3, HCA57, hTid 1, TID 1, TID1
计算分子量 480 aa, 53 kDa
观测分子量 40 kDa
GenBank蛋白编号BC011855
基因名称 TID1
Gene ID (NCBI) 9093
RRIDAB_2293102
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96EY1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TID1 antibody 11088-1-APDownload protocol
IHC protocol for TID1 antibody 11088-1-APDownload protocol
IP protocol for TID1 antibody 11088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

Role of human HSPE1 for OPA1 processing independent of HSPD1

Authors - Nelson Yeung
View Article
humanWB

Nat Commun

Proteogenomic characterization of skull-base chordoma

Authors - Qilin Zhang
  • KD Validated
View Article