DNAJB14 Polyclonal antibody

DNAJB14 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16501-1-AP

Print datasheet

Synonyms

UNQ9427/PRO34683, PRO34683, EGNR9427



经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, human testis tissue
Positive IP detected inJurkat cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16501-1-AP targets DNAJB14 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Immunogen DNAJB14 fusion protein Ag9668 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily B, member 14
Synonyms UNQ9427/PRO34683, PRO34683, EGNR9427
Calculated Molecular Weight 379 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC022248
Gene Symbol DNAJB14
Gene ID (NCBI) 79982
RRIDAB_2094414
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TBM8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DNAJB14 antibody 16501-1-APDownload protocol
IF protocol for DNAJB14 antibody 16501-1-APDownload protocol
IP protocol for DNAJB14 antibody 16501-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry.

Authors - Parikshit Bagchi
humanWB

PLoS Pathog

A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.

Authors - Christopher Paul Walczak
WB

PLoS Pathog

A Non-enveloped Virus Hijacks Host Disaggregation Machinery to Translocate across the Endoplasmic Reticulum Membrane.

Authors - Madhu Sudhan Ravindran
humanIF

J Virol

The ER membrane J protein C18 executes a distinct role in promoting SV40 membrane penetration.

Authors - Parikshit Bagchi
humanWB

J Virol

SGTA-dependent regulation of Hsc70 promotes cytosol entry of SV40 from the ER.

Authors - Allison Dupzyk
humanWB

J Virol

Ubqln4 facilitates ER-to-cytosol escape of a nonenveloped virus during infection.

Authors - Xiaofang Liu