DNAJB14 Polyclonal antibody
DNAJB14 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, HeLa cells, human testis tissue |
Positive IP detected in | Jurkat cells |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 7 publications below |
IF | See 3 publications below |
产品信息
16501-1-AP targets DNAJB14 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, monkey |
Immunogen | DNAJB14 fusion protein Ag9668 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | DnaJ (Hsp40) homolog, subfamily B, member 14 |
Synonyms | UNQ9427/PRO34683, PRO34683, EGNR9427 |
Calculated Molecular Weight | 379 aa, 43 kDa |
Observed Molecular Weight | 43 kDa |
GenBank Accession Number | BC022248 |
Gene Symbol | DNAJB14 |
Gene ID (NCBI) | 79982 |
RRID | AB_2094414 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8TBM8 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
---|---|
WB protocol for DNAJB14 antibody 16501-1-AP | Download protocol |
IF protocol for DNAJB14 antibody 16501-1-AP | Download protocol |
IP protocol for DNAJB14 antibody 16501-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry. | ||
PLoS Pathog A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum. | ||
PLoS Pathog A Non-enveloped Virus Hijacks Host Disaggregation Machinery to Translocate across the Endoplasmic Reticulum Membrane. | ||
J Virol The ER membrane J protein C18 executes a distinct role in promoting SV40 membrane penetration. | ||
J Virol Ubqln4 facilitates ER-to-cytosol escape of a nonenveloped virus during infection. |