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  • KD/KO Validated

DNAJB4 Polyclonal antibody

DNAJB4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 13064-1-AP

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Synonyms

DjB4, DNAJB4, DNAJW, Heat shock protein 40 homolog, HLJ1, HSP40 homolog, Human liver DnaJ like protein



经过测试的应用

Positive WB detected inHeLa cells, mouse serum, mouse skeletal muscle, rat skeletal muscle
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13064-1-AP targets DNAJB4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen DNAJB4 fusion protein Ag3756 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily B, member 4
Synonyms DjB4, DNAJB4, DNAJW, Heat shock protein 40 homolog, HLJ1, HSP40 homolog, Human liver DnaJ like protein
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC034721
Gene Symbol DNAJB4
Gene ID (NCBI) 11080
RRIDAB_2094422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UDY4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DNAJB4 antibody 13064-1-APDownload protocol
IHC protocol for DNAJB4 antibody 13064-1-APDownload protocol
IF protocol for DNAJB4 antibody 13064-1-APDownload protocol
IP protocol for DNAJB4 antibody 13064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

Acta Neuropathol

Distinctive chaperonopathy in skeletal muscle associated with the dominant variant in DNAJB4

Authors - Michio Inoue
mouseWB

Int J Mol Sci

Genetic Deletion of HLJ1 Does Not Affect Blood Coagulation in Mice.

Authors - Man-Chen Hsu
  • KO Validated
humanWB

OMICS

HLJ1 (DNAJB4) Gene Is a Novel Biomarker Candidate in Breast Cancer.

Authors - Tolga Acun
humanWB,IHC

Int J Clin Exp Pathol

HLJ1 is a novel biomarker for colorectal carcinoma progression and overall patient survival.

Authors - Yong Liu
mouseWB

Mol Ther Nucleic Acids

DNAJB6 isoform specific knockdown: Therapeutic potential for limb girdle muscular dystrophy D1

Authors - Andrew R Findlay
humanIHC,WB

Discov Oncol

DNAJB4 suppresses breast cancer progression and promotes tumor immunity by regulating the Hippo signaling pathway

Authors - Yanru Chen