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验证数据展示

  • WB analysis using 67966-1-Ig

    WB analysis using 67966-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 67966-1-Ig

    IHC staining of human pancreas cancer using 67966-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 67966-1-Ig

    IF Staining of A431 using 67966-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using DNAJB4 antibody (67966-1-Ig, Clone: 1F3H7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis of mouse heart using 67966-1-Ig

    WB analysis of mouse heart using 67966-1-Ig

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 67966-1-Ig

    IHC staining of human pancreas cancer using 67966-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DNAJB4 Monoclonal antibody

DNAJB4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67966-1-Ig

产品说明书

CloneNo. 1F3H7

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig, rabbit, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

DjB4, DNAJB4, DNAJW, Heat shock protein 40 homolog, HLJ1, HSP40 homolog, Human liver DnaJ like protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, mouse heart tissue, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, pig heart, rabbit heart
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67966-1-Ig targets DNAJB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig, rabbit, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, pig, rabbit, mouse
免疫原 DNAJB4 fusion protein Ag3756 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily B, member 4
别名 DjB4, DNAJB4, DNAJW, Heat shock protein 40 homolog, HLJ1, HSP40 homolog, Human liver DnaJ like protein
计算分子量 38 kDa
观测分子量38 kDa
GenBank蛋白编号BC034721
基因名称 DNAJB4
Gene ID (NCBI) 11080
RRIDAB_2918717
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UDY4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DNAJB4 antibody 67966-1-IgDownload protocol
IHC protocol for DNAJB4 antibody 67966-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols