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DNAJB4 Monoclonal antibody

DNAJB4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F3H7

Cat no : 67966-1-Ig

Print datasheet

Synonyms

DjB4, DNAJB4, DNAJW, Heat shock protein 40 homolog, HLJ1, HSP40 homolog, Human liver DnaJ like protein

验证数据展示

  • WB analysis using 67966-1-Ig

    WB analysis using 67966-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 67966-1-Ig

    IHC staining of human pancreas cancer using 67966-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 67966-1-Ig

    IF Staining of A431 using 67966-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using DNAJB4 antibody (67966-1-Ig, Clone: 1F3H7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis of mouse heart using 67966-1-Ig

    WB analysis of mouse heart using 67966-1-Ig

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 67966-1-Ig

    IHC staining of human pancreas cancer using 67966-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, mouse heart tissue, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, pig heart, rabbit heart
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67966-1-Ig targets DNAJB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig, rabbit, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, pig, rabbit, mouse
Immunogen DNAJB4 fusion protein Ag3756 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily B, member 4
Synonyms DjB4, DNAJB4, DNAJW, Heat shock protein 40 homolog, HLJ1, HSP40 homolog, Human liver DnaJ like protein
Calculated Molecular Weight 38 kDa
Observed Molecular Weight38 kDa
GenBank Accession NumberBC034721
Gene Symbol DNAJB4
Gene ID (NCBI) 11080
RRIDAB_2918717
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UDY4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DNAJB4 antibody 67966-1-IgDownload protocol
IHC protocol for DNAJB4 antibody 67966-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols