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DNAJB5 Polyclonal antibody

DNAJB5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16453-1-AP

Print datasheet

Synonyms

KIAA1045, Hsc40, Heat shock protein Hsp40-3, Heat shock protein Hsp40-2, Heat shock protein Hsp40 3

验证数据展示

  • WB analysis of rat heart using 16453-1-AP

    WB analysis of rat heart using 16453-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 16453-1-AP

    IP experiment of HepG2 using 16453-1-AP

    IP result of anti-DNAJB5 (IP:16453-1-AP, 4ug; Detection:16453-1-AP 1:500) with HepG2 cells lysate 3300 ug.

  • IHC staining of human heart using 16453-1-AP

    IHC staining of human heart using 16453-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 16453-1-AP

    IHC staining of human heart using 16453-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 16453-1-AP

    IF Staining of HepG2 using 16453-1-AP

    Immunofluorescent analysis of HepG2 cells using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inrat heart tissue, HeLa cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman heart tissue, human kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16453-1-AP targets DNAJB5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DNAJB5 fusion protein Ag9341 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily B, member 5
Synonyms KIAA1045, Hsc40, Heat shock protein Hsp40-3, Heat shock protein Hsp40-2, Heat shock protein Hsp40 3
Calculated Molecular Weight 386 aa, 44 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC012115
Gene Symbol DNAJB5
Gene ID (NCBI) 25822
RRIDAB_2094724
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75953
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJB5, also named as Hsc40, is an Hsp40 Family Protein with MW 39-40 kDa. It mediates antihypertrophic effects of Thioredoxin1 in the heart. This protein has some isoforms with MW 39 kDa and 47-51 kDa.

实验方案

Product Specific Protocols
WB protocol for DNAJB5 antibody 16453-1-APDownload protocol
IHC protocol for DNAJB5 antibody 16453-1-APDownload protocol
IF protocol for DNAJB5 antibody 16453-1-APDownload protocol
IP protocol for DNAJB5 antibody 16453-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols