• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of rat heart using 16453-1-AP

    WB analysis of rat heart using 16453-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 16453-1-AP

    IP experiment of HepG2 using 16453-1-AP

    IP result of anti-DNAJB5 (IP:16453-1-AP, 4ug; Detection:16453-1-AP 1:500) with HepG2 cells lysate 3300 ug.

  • IHC staining of human heart using 16453-1-AP

    IHC staining of human heart using 16453-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 16453-1-AP

    IHC staining of human heart using 16453-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 16453-1-AP

    IF Staining of HepG2 using 16453-1-AP

    Immunofluorescent analysis of HepG2 cells using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

DNAJB5 Polyclonal antibody

DNAJB5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16453-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

KIAA1045, Hsc40, Heat shock protein Hsp40-3, Heat shock protein Hsp40-2, Heat shock protein Hsp40 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inrat heart tissue, HeLa cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman heart tissue, human kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16453-1-AP targets DNAJB5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DNAJB5 fusion protein Ag9341 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily B, member 5
别名 KIAA1045, Hsc40, Heat shock protein Hsp40-3, Heat shock protein Hsp40-2, Heat shock protein Hsp40 3
计算分子量 386 aa, 44 kDa
观测分子量 39 kDa
GenBank蛋白编号BC012115
基因名称 DNAJB5
Gene ID (NCBI) 25822
RRIDAB_2094724
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75953
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJB5, also named as Hsc40, is an Hsp40 Family Protein with MW 39-40 kDa. It mediates antihypertrophic effects of Thioredoxin1 in the heart. This protein has some isoforms with MW 39 kDa and 47-51 kDa.

实验方案

Product Specific Protocols
WB protocol for DNAJB5 antibody 16453-1-APDownload protocol
IHC protocol for DNAJB5 antibody 16453-1-APDownload protocol
IF protocol for DNAJB5 antibody 16453-1-APDownload protocol
IP protocol for DNAJB5 antibody 16453-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols