验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, Y79 cells, mouse brain tissue, mouse testis tissue, rat brain tissue, Jurkat cells, SH-SY5Y cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11707-1-AP targets DNAJB6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | DNAJB6 fusion protein Ag2258 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DnaJ (Hsp40) homolog, subfamily B, member 6 |
| 别名 | HSJ2, HSJ 2, HHDJ1, Heat shock protein J2, DnaJ |
| 计算分子量 | 241 aa, 27 kDa |
| 观测分子量 | 27 kDa, 38 kDa |
| GenBank蛋白编号 | BC000177 |
| 基因名称 | DNAJB6 |
| Gene ID (NCBI) | 10049 |
| RRID | AB_2230757 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75190 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID:20159555).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJB6 antibody 11707-1-AP | Download protocol |
| IHC protocol for DNAJB6 antibody 11707-1-AP | Download protocol |
| IF protocol for DNAJB6 antibody 11707-1-AP | Download protocol |
| IP protocol for DNAJB6 antibody 11707-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neurobiol Dis DNAJB6 suppresses alpha-synuclein induced pathology in an animal model of Parkinson's disease. | ||
iScience Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis. | ||
Mol Carcinog Overexpression of DNAJB6 promotes colorectal cancer cell invasion through an IQGAP1/ERK-dependent signaling pathway.
| ||
STAR Protoc Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse. | ||
Cancers (Basel) Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin |