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验证数据展示

  • WB analysis of Jurkat using 66587-1-Ig (same clone as 66587-1-PBS)

    WB analysis of Jurkat using 66587-1-Ig (same clone as 66587-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66587-1-Ig (same clone as 66587-1-PBS)

    IHC staining of human testis using 66587-1-Ig (same clone as 66587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66587-1-Ig (same clone as 66587-1-PBS)

    WB analysis of HeLa using 66587-1-Ig (same clone as 66587-1-PBS)

    WB result of DNAJB6 antibody (66587-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJB6 transfected HeLa cells. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DNAJB6 Monoclonal antibody, PBS Only

DNAJB6 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66587-1-PBS

Print datasheet

CloneNo. 1A1D2

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, Indirect ELISA

Synonyms

DJ4, DnaJ, DNAJB6, Heat shock protein J2, HHDJ1, HSJ 2, HSJ2, MRJ, MSJ 1, MSJ1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

66587-1-PBS targets DNAJB6 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen DNAJB6 fusion protein Ag2258 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily B, member 6
Synonyms DJ4, DnaJ, DNAJB6, Heat shock protein J2, HHDJ1, HSJ 2, HSJ2, MRJ, MSJ 1, MSJ1
Calculated Molecular Weight 241 aa, 27 kDa
Observed Molecular Weight 27 kDa, 38 kDa
GenBank Accession NumberBC000177
Gene Symbol DNAJB6
Gene ID (NCBI) 10049
RRIDAB_2881947
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75190
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID: 20159555). The antibody 66587-1-Ig recognizes both isoforms.