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验证数据展示

  • WB analysis of Jurkat using 66587-1-Ig (same clone as 66587-1-PBS)

    WB analysis of Jurkat using 66587-1-Ig (same clone as 66587-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66587-1-Ig (same clone as 66587-1-PBS)

    IHC staining of human testis using 66587-1-Ig (same clone as 66587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66587-1-Ig (same clone as 66587-1-PBS)

    WB analysis of HeLa using 66587-1-Ig (same clone as 66587-1-PBS)

    WB result of DNAJB6 antibody (66587-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJB6 transfected HeLa cells. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

DNAJB6 Monoclonal antibody, PBS Only

DNAJB6 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66587-1-PBS

产品说明书

CloneNo. 1A1D2

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IHC, Indirect ELISA

别名

DJ4, DnaJ, DNAJB6, Heat shock protein J2, HHDJ1, HSJ 2, HSJ2, MRJ, MSJ 1, MSJ1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66587-1-PBS targets DNAJB6 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 DNAJB6 fusion protein Ag2258 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily B, member 6
别名 DJ4, DnaJ, DNAJB6, Heat shock protein J2, HHDJ1, HSJ 2, HSJ2, MRJ, MSJ 1, MSJ1
计算分子量 241 aa, 27 kDa
观测分子量 27 kDa, 38 kDa
GenBank蛋白编号BC000177
基因名称 DNAJB6
Gene ID (NCBI) 10049
RRIDAB_2881947
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO75190
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID: 20159555). The antibody 66587-1-Ig recognizes both isoforms.