DNAJB8 Polyclonal antibody

DNAJB8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17071-1-AP

Print datasheet

Synonyms

DJ6



经过测试的应用

Positive WB detected inHeLa cells, human brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17071-1-AP targets DNAJB8 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, pig
Immunogen DNAJB8 fusion protein Ag10899 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily B, member 8
Synonyms DJ6
Calculated Molecular Weight 232 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC029521
Gene Symbol DNAJB8
Gene ID (NCBI) 165721
RRIDAB_2094743
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NHS0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJB8, a member of the DNAJ/HSP40 family of proteins, plays a crucial role in regulating chaperone HSP70 activity. It is known for its ability to suppress the aggregation of polyglutamine proteins through its C-terminal tail. Furthermore, DNAJB8 has been shown to inhibit TP53 through ubiquitin degradation in human colon cancer by directly interacting and upregulating MDR1, leading to increased oxaliplatin resistance. High levels of DNAJB8 in both colon tissues and serum are correlated with worse overall survival of colon cancer patients, suggesting that DNAJB8 level in serum sEV may serve as a potential biomarker for colon cancer and a promising therapeutic target for oxaliplatin-resistant cancer.

实验方案

Product Specific Protocols
WB protocol for DNAJB8 antibody 17071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

iScience

Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.

Authors - Qianxing Zou
mouseWB,IF

STAR Protoc

Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse.

Authors - Qianxing Zou
pigWB

Vet Microbiol

The heat shock protein DNAJB8 inhibits pseudorabies virus replication by autophagy

Authors - Xiaoyong Chen