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DNAJC1 Polyclonal antibody

DNAJC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12362-1-AP

Print datasheet

Synonyms

DnaJ protein homolog MTJ1, DNAJC1, DNAJL1, ERdj1, HTJ1, MTJ1

验证数据展示

  • WB analysis using 12362-1-AP

    WB analysis using 12362-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12362-1-AP (DNAJC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 12362-1-AP

    WB analysis of A549 using 12362-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12362-1-AP (DNAJC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of Raji using 12362-1-AP

    IP experiment of Raji using 12362-1-AP

    IP result of anti-DNAJC1 (IP:12362-1-AP, 4ug; Detection:12362-1-AP 1:1000) with Raji cells lysate 3200 ug.

  • IHC staining of human breast cancer using 12362-1-AP

    IHC staining of human breast cancer using 12362-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 12362-1-AP

    IHC staining of human breast cancer using 12362-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 12362-1-AP

    IHC staining of human liver cancer using 12362-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 12362-1-AP

    IHC staining of human liver cancer using 12362-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells, A549 cells, Raji cells, NIH/3T3 cells
Positive IP detected inRaji cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12362-1-AP targets DNAJC1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DNAJC1 fusion protein Ag3030 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily C, member 1
Synonyms DnaJ protein homolog MTJ1, DNAJC1, DNAJL1, ERdj1, HTJ1, MTJ1
Calculated Molecular Weight 554 aa, 64 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC030955
Gene Symbol DNAJC1
Gene ID (NCBI) 64215
RRIDAB_2094754
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96KC8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DNAJC1 antibody 12362-1-APDownload protocol
IHC protocol for DNAJC1 antibody 12362-1-APDownload protocol
IP protocol for DNAJC1 antibody 12362-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cancer Res Clin Oncol

DNAJC1 facilitates glioblastoma progression by promoting extracellular matrix reorganization and macrophage infiltration

Authors - Han Zhang
View Article