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DNAJC10 Polyclonal antibody

DNAJC10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Publications

Cat no : 13101-1-AP

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Synonyms

JPDI, ER-resident protein ERdj5, ERdj5, ER resident protein ERdj5, Endoplasmic reticulum DNA J domain-containing protein 5

验证数据展示

WB analysis of HeLa using 13101-1-AP
WB result of DNAJC10 antibody (13101-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJC10 transfected HeLa cells.
WB analysis using 13101-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13101-1-AP (DNAJC10 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 13101-1-AP
IP result of anti-DNAJC10 (IP:13101-1-AP, 4ug; Detection:13101-1-AP 1:500) with HeLa cells lysate 1600 ug.
IHC staining of human pancreas cancer using 13101-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13101-1-AP (DNAJC10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 13101-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13101-1-AP (DNAJC10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 13101-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13101-1-AP (DNAJC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 13101-1-AP

Size

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经过测试的应用

Positive WB detected in	HeLa cells, HepG2 cells, mouse liver tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human pancreas cancer tissue, human stomach tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 12 publications below
IHC	See 1 publications below
IF	See 2 publications below
CoIP	See 1 publications below

产品信息

13101-1-AP targets DNAJC10 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IHC, CoIP, IF
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Immunogen	DNAJC10 fusion protein Ag3737 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	DnaJ (Hsp40) homolog, subfamily C, member 10
Synonyms	JPDI, ER-resident protein ERdj5, ERdj5, ER resident protein ERdj5, Endoplasmic reticulum DNA J domain-containing protein 5
Calculated Molecular Weight	793 aa, 91 kDa
Observed Molecular Weight	80-90 kDa
GenBank Accession Number	BC034713
Gene Symbol	DNAJC10
Gene ID (NCBI)	54431
RRID	AB_10638440
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IXB1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

DNAJC10(DnaJ homolog subfamily C member 10)is also named as ERDJ5. The human ER-resident protein (ERdj5) ubiquitously expresses and is abundant in secretory tissues such as pancreas and testis and it may be involved in assisting protein folding and quality control in the ER(PMID:12411443). The deduced 793-amino acid protein has a calculated molecular mass of about 91 kD. ERDJ5 contains an N-terminal hydrophobic sequence, followed by a type III DnaJ domain, 4 thioredoxin-like domains, and a C-terminal KDEL endoplasmic reticulum (ER) retention signal. It can be modified by N-linked glycosylation(PMID:12446677). The ERdj5 antibody displays a higher affinity for endogenous ERdj5u.

实验方案

Product Specific Protocols
WB protocol for DNAJC10 antibody 13101-1-AP	Download protocol
IHC protocol for DNAJC10 antibody 13101-1-AP	Download protocol
IF protocol for DNAJC10 antibody 13101-1-AP	Download protocol
IP protocol for DNAJC10 antibody 13101-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	PLoS Pathog A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum. Authors - Christopher Paul Walczak View Article
human	WB,CoIP	Mol Cell Proteomics Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis Authors - Madison T. Wright View Article
human	WB	Diabetes Unbiased Profiling of the Human Proinsulin Biosynthetic Interaction Network Reveals a Role For Peroxiredoxin 4 in Proinsulin Folding. Authors - Duc T Tran View Article
human	WB	Mol Cell Proteomics Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis. Authors - Madison T Wright View Article
human	WB	Mol Biol Cell The ERdj5-Sel1L complex facilitates cholera toxin retrotranslocation. Authors - Williams Jeffrey M JM View Article
human	WB	Cell Stress Chaperones Amyotrophic lateral sclerosis (ALS) and Alzheimer's disease (AD) are characterised by differential activation of ER stress pathways: focus on UPR target genes. Authors - L Montibeller View Article

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DNAJC10 Polyclonal antibody

DNAJC10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

验证数据展示

WB analysis of HeLa using 13101-1-AP

WB analysis using 13101-1-AP

IP experiment of HeLa using 13101-1-AP

IHC staining of human pancreas cancer using 13101-1-AP

IHC staining of human pancreas cancer using 13101-1-AP

IF Staining of HeLa using 13101-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.

Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis

Unbiased Profiling of the Human Proinsulin Biosynthetic Interaction Network Reveals a Role For Peroxiredoxin 4 in Proinsulin Folding.

Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis.

The ERdj5-Sel1L complex facilitates cholera toxin retrotranslocation.

Amyotrophic lateral sclerosis (ALS) and Alzheimer's disease (AD) are characterised by differential activation of ER stress pathways: focus on UPR target genes.