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  • KD/KO Validated

DNAJC19 Polyclonal antibody

DNAJC19 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12096-1-AP

Print datasheet

Synonyms

TIMM14, TIM14



经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, human lung tissue
Positive IP detected inmouse heart tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12096-1-AP targets DNAJC19 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DNAJC19 fusion protein Ag2739 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily C, member 19
Synonyms TIMM14, TIM14
Calculated Molecular Weight 116 aa, 13 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC009702
Gene Symbol DNAJC19
Gene ID (NCBI) 131118
RRIDAB_2094914
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96DA6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJC19 is a mitochondrial cochaperone that interacts with HSP70 chaperones through its conserved J-domain. As a transmembrane protein, DNAJC19 is strongly associated with the inner mitochondrial membrane. Mutations in DNAJC19 cause dilated cardiomyopathy with ataxia (DCMA). Recently DNAJC19 has been reported as interactor of PHB complex to regulate cardiolipin remodeling, which addressed the link of DNAJC19 to cardiomyopathy. This antibody is specific to DNAJC19 and had been tested by siRNA.(24856930)

实验方案

Product Specific Protocols
WB protocol for DNAJC19 antibody 12096-1-APDownload protocol
IF protocol for DNAJC19 antibody 12096-1-APDownload protocol
IP protocol for DNAJC19 antibody 12096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.

Authors - Shotaro Saita
humanWB

Cell Metab

DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.

Authors - Ricarda Richter-Dennerlein
  • KD Validated
humanWB

Hum Mol Genet

Defining functional classes of Barth syndrome mutation in humans.

Authors - Ya-Wen Lu
human

Stem Cell Res

Generation of two patient-derived iPSC lines from siblings (LIBUCi001-A and LIBUCi002-A) and a genetically modified iPSC line (JMUi001-A-1) to mimic dilated cardiomyopathy with ataxia (DCMA) caused by a homozygous DNAJC19 mutation.

Authors - Anna Janz
humanWB

Cell Rep

The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism.

Authors - Michelle Grace Acoba
humanWB

Cell Rep

LPGAT1 controls MEGDEL syndrome by coupling phosphatidylglycerol remodeling with mitochondrial transport

Authors - Haoran Sun