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验证数据展示

  • WB analysis of HEK-293 using 23411-1-AP

    WB analysis of HEK-293 using 23411-1-AP

    WB results of DNAJC21 antibody (23411-1-AP, 1:2000) with si-control and si-DNAJC21 transfected HEK293 cells.

  • IP experiment of HEK-293 using 23411-1-AP

    IP experiment of HEK-293 using 23411-1-AP

    IP result of anti-DNAJC21 (IP:23411-1-AP, 4ug; Detection:23411-1-AP 1:1000) with HEK-293 cells lysate 2700ug.

  • IHC staining of human pancreas using 23411-1-AP

    IHC staining of human pancreas using 23411-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23411-1-AP (DNAJC21 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas using 23411-1-AP

    IHC staining of human pancreas using 23411-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23411-1-AP (DNAJC21 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 23411-1-AP

    IF Staining of HeLa using 23411-1-AP

    Immunofluorescent analysis of HeLa cells using 23411-1-AP (DNAJC21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 23411-1-AP

    WB analysis using 23411-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

DNAJC21 Polyclonal antibody

DNAJC21 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 23411-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Synonyms

Protein GS3, GS3, DNAJA5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, HeLa cells, rat brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23411-1-AP targets DNAJC21 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DNAJC21 fusion protein Ag20003 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily C, member 21
Synonyms Protein GS3, GS3, DNAJA5
Calculated Molecular Weight 531 aa, 58 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC107577
Gene Symbol DNAJC21
Gene ID (NCBI) 134218
RRIDAB_2879274
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ5F1R6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJC21 is a ubiquitously expressed DnaJ (heat shock protein 40) chaperone protein. Recently DNAJC21 was reported to be implicated in rRNA biogenesis. Loss of DNAJC21 induced abnormal cell morphology and inhibited cell growth. Mutations in DNAJC21 cause bone marrow failure syndrome. (27346687)

实验方案

Product Specific Protocols
WB protocol for DNAJC21 antibody 23411-1-APDownload protocol
IHC protocol for DNAJC21 antibody 23411-1-APDownload protocol
IF protocol for DNAJC21 antibody 23411-1-APDownload protocol
IP protocol for DNAJC21 antibody 23411-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Stem Cell

HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.

Authors - Kaosheng Lv
View Article
humanWB,IF

Am J Hum Genet

DNAJC21 Mutations Link a Cancer-Prone Bone Marrow Failure Syndrome to Corruption in 60S Ribosome Subunit Maturation.

Authors - Hemanth Tummala
  • KD Validated
View Article