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DNAJC25 Polyclonal antibody

DNAJC25 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26229-1-AP

Print datasheet

Synonyms

DNAJC25

验证数据展示

  • WB analysis using 26229-1-AP

    WB analysis using 26229-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26229-1-AP (DNAJC25 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 26229-1-AP

    IHC staining of human liver using 26229-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26229-1-AP (DNAJC25 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 26229-1-AP

    IHC staining of human liver using 26229-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26229-1-AP (DNAJC25 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 26229-1-AP

    IF Staining of U2OS using 26229-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using DNAJC25 antibody (26229-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, human placenta tissue, mouse liver tisue, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26229-1-AP targets DNAJC25 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DNAJC25 fusion protein Ag23760 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily C , member 25
Synonyms DNAJC25
Observed Molecular Weight36-42 kDa
GenBank Accession NumberBC048318
Gene Symbol DNAJC25
Gene ID (NCBI) 548645
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H1X3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DnaJ heat shock protein family (Hsp40) member C25 (DNAJC25), which belongs to the HSP40 subfamily C, is localized to the cytoplasm. DNAJC25 had a markedly high expression level in liver tissues and was significantly downregulated in liver cancer tissues compared with the adjacent normal tissues (PMID: 23205125).

实验方案

Product Specific Protocols
WB protocol for DNAJC25 antibody 26229-1-APDownload protocol
IHC protocol for DNAJC25 antibody 26229-1-APDownload protocol
IF protocol for DNAJC25 antibody 26229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols