DNAJC3 Polyclonal antibody
DNAJC3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | human colon cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
The immunogen of 26721-1-AP is DNAJC3 Fusion Protein expressed in E. coli.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | DNAJC3 fusion protein Ag24966 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | DnaJ (Hsp40) homolog, subfamily C, member 3 |
| Synonyms | DNAJC3, HP58, P58, P58IPK, PRKRI, Protein kinase inhibitor p58 |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | BC047936 |
| Gene Symbol | DNAJC3 |
| Gene ID (NCBI) | 5611 |
| RRID | AB_2880613 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13217 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNAJC3, also known as P58,HP58 and P58IPK, belongs to heat shock protein family,which is a kind of conservative molecules that expresses extensively in cells of human and animals and protects cells against all kinds of damage. As a cochaperone of BiP that an HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum(ER), DNAJC3 plays a crucial role in ER protein folding and the UPR(unfolded protein response) (PMID:10597629; PMID:25466870). Studies reported that DNAJC3 related several diseases such as osteosarcoma, widespread neurodegeneration, diabetes mellitus, sensorimotor neuropathy, ataxia, sensorineural hearing loss and short body stature(PMID:30652414; PMID:28940199).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJC3 antibody 26721-1-AP | Download protocol |
| IHC protocol for DNAJC3 antibody 26721-1-AP | Download protocol |
| IF protocol for DNAJC3 antibody 26721-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
FEBS Open Bio A systems biological analysis of the ATF4-GADD34-CHOP regulatory triangle upon endoplasmic reticulum stress |
