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DNAJC4 Polyclonal antibody

DNAJC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13853-1-AP

Print datasheet

Synonyms

DANJC4, DnaJ like protein HSPF2, DNAJC4, HSPF2, MCG18

验证数据展示

  • IHC staining of human breast cancer using 13853-1-AP

    IHC staining of human breast cancer using 13853-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13853-1-AP (DNAJC4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human breast cancer using 13853-1-AP

    IHC staining of human breast cancer using 13853-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13853-1-AP (DNAJC4 antibody) at dilution of 1:100 (under 40x lens).

  • WB analysis of MCF-7 using 13853-1-AP

    WB analysis of MCF-7 using 13853-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 13853-1-AP (DNAJC4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13853-1-AP targets DNAJC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DNAJC4 fusion protein Ag4826 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily C, member 4
Synonyms DANJC4, DnaJ like protein HSPF2, DNAJC4, HSPF2, MCG18
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC044584
Gene Symbol DNAJC4
Gene ID (NCBI) 3338
RRIDAB_2095224
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NNZ3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DNAJC4 antibody 13853-1-APDownload protocol
IHC protocol for DNAJC4 antibody 13853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols