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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis of mouse testis using 17364-1-AP

    WB analysis of mouse testis using 17364-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17364-1-AP (DNAJC5B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 17364-1-AP

    IF Staining of HepG2 using 17364-1-AP

    Immunofluorescent analysis of HepG2 cells, using DNAJC5B antibody 17364-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of mouse testis using 17364-1-AP

    IP experiment of mouse testis using 17364-1-AP

    IP result of anti-DNAJC5B (IP:17364-1-AP, 3ug; Detection:17364-1-AP 1:1500) with mouse testis tissue lysate 4000ug.

DNAJC5B Polyclonal antibody

DNAJC5B Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 17364-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

Cysteine-string protein isoform beta, CSP-beta, CSP beta, Beta cysteine string protein, Beta CSP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17364-1-AP targets DNAJC5B in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DNAJC5B fusion protein Ag10744 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily C, member 5 beta
别名 Cysteine-string protein isoform beta, CSP-beta, CSP beta, Beta cysteine string protein, Beta CSP
计算分子量 199 aa, 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC016742
基因名称 DNAJC5B
Gene ID (NCBI) 85479
RRIDAB_2095062
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UF47
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJC5B encodes cysteine string proteins (CSP) beta. CSPs belong to the DnaJ-like chaperone family and play an important role in regulated exocytosis in neurons and endocrine cells. Mammals express three CSP proteins, α, β, γ. In contrast to the CSPα which is ubiquitously expressed, CSPβ is restricted to testis and auditory hair cell neurons. The predicted MW of DNAJC5B is around 22 kDa, while some modified forms exist (PMID: 17034881)

实验方案

Product Specific Protocols
WB protocol for DNAJC5B antibody 17364-1-APDownload protocol
IF protocol for DNAJC5B antibody 17364-1-APDownload protocol
IP protocol for DNAJC5B antibody 17364-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols