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验证数据展示

  • WB analysis of HeLa using 21941-1-AP

    WB analysis of HeLa using 21941-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis using 21941-1-AP

    WB analysis using 21941-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 21941-1-AP

    IP experiment of mouse brain using 21941-1-AP

    IP result of anti-DNAJC6/AUXILIN (IP:21941-1-AP, 4ug; Detection:21941-1-AP 1:2000) with mouse brain tissue lysate 4000 ug.

  • IHC staining of human brain using 21941-1-AP

    IHC staining of human brain using 21941-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21941-1-AP (DNAJC6/AUXILIN antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 21941-1-AP

    IHC staining of human brain using 21941-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21941-1-AP (DNAJC6/AUXILIN antibody at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 21941-1-AP

    IF Staining of HeLa using 21941-1-AP

    Immunofluorescent analysis of HeLa cells using 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

DNAJC6/AUXILIN Polyclonal antibody

DNAJC6/AUXILIN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21941-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

AUXILIN, DJC6, DNAJC6, DNAJC6/AUXILIN, KIAA0473

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, human brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21941-1-AP targets DNAJC6/AUXILIN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 DNAJC6/AUXILIN fusion protein Ag16612 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily C, member 6
别名 AUXILIN, DJC6, DNAJC6, DNAJC6/AUXILIN, KIAA0473
计算分子量 970 aa, 106 kDa
观测分子量 100-120 kDa, 86 kDa
GenBank蛋白编号BC109279
基因名称 DNAJC6/AUXILIN
Gene ID (NCBI) 9829
RRIDAB_2878951
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75061
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJC6, also named AUXILIN, belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. It is a neuronal protein that functions specifically in the pathway of clathrin-mediated endocytosis. It shares homology with GAK, and the two proteins act as cochaperones to support the HSC70 -dependent clathrin uncoating of clathrin-coated vesicles (PMID: 2016009). DNAJC6 is expressed in various brain regions, including cerebellum, corpus callosum, cortex, striatum, brainstem, pons, putamen, spinal cord and substantia nigra (PMID: 23211418).

实验方案

Product Specific Protocols
WB protocol for DNAJC6/AUXILIN antibody 21941-1-APDownload protocol
IHC protocol for DNAJC6/AUXILIN antibody 21941-1-APDownload protocol
IF protocol for DNAJC6/AUXILIN antibody 21941-1-APDownload protocol
IP protocol for DNAJC6/AUXILIN antibody 21941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Sci Rep

Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity.

Authors - Ermes Candiello
View Article
humanWB

Cancers (Basel)

Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin

Authors - Mohamed Alalem
View Article