DNAJC6/AUXILIN Polyclonal antibody

DNAJC6/AUXILIN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 21941-1-AP

Print datasheet

Synonyms

AUXILIN, DJC6, DNAJC6, DNAJC6/AUXILIN, KIAA0473



经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, human brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 21941-1-AP is DNAJC6/AUXILIN Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen DNAJC6/AUXILIN fusion protein Ag16612 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily C, member 6
Synonyms AUXILIN, DJC6, DNAJC6, DNAJC6/AUXILIN, KIAA0473
Calculated Molecular Weight 970 aa, 106 kDa
Observed Molecular Weight 100-120 kDa, 86 kDa
GenBank Accession NumberBC109279
Gene Symbol DNAJC6/AUXILIN
Gene ID (NCBI) 9829
RRIDAB_2878951
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75061
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJC6, also named AUXILIN, belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. It is a neuronal protein that functions specifically in the pathway of clathrin-mediated endocytosis. It shares homology with GAK, and the two proteins act as cochaperones to support the HSC70 -dependent clathrin uncoating of clathrin-coated vesicles (PMID: 2016009). DNAJC6 is expressed in various brain regions, including cerebellum, corpus callosum, cortex, striatum, brainstem, pons, putamen, spinal cord and substantia nigra (PMID: 23211418).

实验方案

Product Specific Protocols
WB protocol for DNAJC6/AUXILIN antibody 21941-1-APDownload protocol
IHC protocol for DNAJC6/AUXILIN antibody 21941-1-APDownload protocol
IF protocol for DNAJC6/AUXILIN antibody 21941-1-APDownload protocol
IP protocol for DNAJC6/AUXILIN antibody 21941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Sci Rep

Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity.

Authors - Ermes Candiello
humanWB

Cancers (Basel)

Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin

Authors - Mohamed Alalem