验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HEK-293 cells, Jurkat cells, moust testis |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11090-1-AP targets DNAJC7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | DNAJC7 fusion protein Ag1572 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DnaJ (Hsp40) homolog, subfamily C, member 7 |
| 别名 | CCRP, DANJC7, DNAJC 7, TPR2, TTC2 |
| 计算分子量 | 56 kDa |
| 观测分子量 | 56 kDa |
| GenBank蛋白编号 | BC011837 |
| 基因名称 | DNAJC7 |
| Gene ID (NCBI) | 7266 |
| RRID | AB_2293179 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99615 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DnaJC7, otherwise referred to as Tpr2 or CCRP, is a conserved, type of J protein that is highly expressed within the brain. DnaJC7 has been found to modulate the flow of substrates from Hsp70 to Hsp90, providing a control mechanism in proteins such as glucocorticoids and progesterone receptor.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJC7 antibody 11090-1-AP | Download protocol |
| IHC protocol for DNAJC7 antibody 11090-1-AP | Download protocol |
| IF protocol for DNAJC7 antibody 11090-1-AP | Download protocol |
| IP protocol for DNAJC7 antibody 11090-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun DnaJC7 binds natively folded structural elements in tau to inhibit amyloid formation. | ||
Biochem Pharmacol Indirect activation of constitutive androstane receptor in three-dimensionally cultured HepG2 cells.
| ||
Biochem Biophys Res Commun Co-chaperon DnaJC7/TPR2 enhances p53 stability and activity through blocking the complex formation between p53 and MDM2. | ||
Cancers (Basel) Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin |
