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验证数据展示

  • WB analysis of HEK-293 using 25444-1-AP

    WB analysis of HEK-293 using 25444-1-AP

    WB result of DNAJC9 antibody (25444-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJC9 transfected HEK-293 cells.

  • IHC staining of human colon cancer using 25444-1-AP

    IHC staining of human colon cancer using 25444-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DNAJC9 antibody included
  • IF Staining of U2OS using 25444-1-AP

    IF Staining of U2OS using 25444-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DNAJC9 antibody (25444-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human breast cancer using 25444-1-AP

    IHC staining of human breast cancer using 25444-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DNAJC9 antibody included
  • WB analysis using 25444-1-AP

    WB analysis using 25444-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25444-1-AP (DNAJC9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 25444-1-AP

    IHC staining of human breast cancer using 25444-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DNAJC9 antibody included
  • Featured Product
  • KD/KO验证

DNAJC9 Polyclonal antibody

DNAJC9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25444-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

SB73, KIAA0974, JDD1, HDJC9, DnaJ protein SB73

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, SH-SY5Y cells, Raji cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, mouse colon tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy DNAJC9 Ready-To-Use IHC Kit. DNAJC9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

25444-1-AP targets DNAJC9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 DNAJC9 fusion protein Ag22123 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily C, member 9
别名 SB73, KIAA0974, JDD1, HDJC9, DnaJ protein SB73
计算分子量 260 aa, 30 kDa
观测分子量 30-35 kDa
GenBank蛋白编号BC136507
基因名称 DNAJC9
Gene ID (NCBI) 23234
RRIDAB_2880084
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WXX5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJC9, DnaJ homolog subfamily C member 9, acts as a dual histone chaperone and heat shock co-chaperone (PMID: 33857403). As a histone chaperone, DNAJC9 forms a co-chaperone complex with MCM2 and histone H3-H4 heterodimers (PMID: 33857403). DNAJC9 also plays a role as co-chaperone of the HSP70 family of molecular chaperone proteins. (PMID: 17182002, PMID: 33857403). DNAJC9 exhibits activity to assemble histones onto DNA (PMID: 33857403). DNAJC9 is an essential protein in many cancer cell types and the levels of the protein correlate with the rates at which cancer cells proliferate.

实验方案

Product Specific Protocols
WB protocol for DNAJC9 antibody 25444-1-APDownload protocol
IHC protocol for DNAJC9 antibody 25444-1-APDownload protocol
IF protocol for DNAJC9 antibody 25444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology.

Authors - Hexiao Shen
View Article
humanWB

bioRxiv

Mechanism of ASF1 Inhibition by CDAN1

Authors - Samantha F Sedor
View Article