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  • Featured Product
  • KD/KO Validated

DNAJC9 Polyclonal antibody

DNAJC9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25444-1-AP

Print datasheet

Synonyms

SB73, KIAA0974, JDD1, HDJC9, DnaJ protein SB73

验证数据展示

  • WB analysis of HEK-293 using 25444-1-AP

    WB analysis of HEK-293 using 25444-1-AP

    WB result of DNAJC9 antibody (25444-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJC9 transfected HEK-293 cells.

  • IHC staining of human colon cancer using 25444-1-AP

    IHC staining of human colon cancer using 25444-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DNAJC9 antibody included
  • IF Staining of U2OS using 25444-1-AP

    IF Staining of U2OS using 25444-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DNAJC9 antibody (25444-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human breast cancer using 25444-1-AP

    IHC staining of human breast cancer using 25444-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DNAJC9 antibody included
  • WB analysis using 25444-1-AP

    WB analysis using 25444-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25444-1-AP (DNAJC9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 25444-1-AP

    IHC staining of human breast cancer using 25444-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DNAJC9 antibody included

经过测试的应用

Positive WB detected inHEK-293 cells, SH-SY5Y cells, Raji cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, mouse colon tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy DNAJC9 Ready-To-Use IHC Kit. DNAJC9 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

25444-1-AP targets DNAJC9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DNAJC9 fusion protein Ag22123 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily C, member 9
Synonyms SB73, KIAA0974, JDD1, HDJC9, DnaJ protein SB73
Calculated Molecular Weight 260 aa, 30 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC136507
Gene Symbol DNAJC9
Gene ID (NCBI) 23234
RRIDAB_2880084
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXX5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJC9, DnaJ homolog subfamily C member 9, acts as a dual histone chaperone and heat shock co-chaperone (PMID: 33857403). As a histone chaperone, DNAJC9 forms a co-chaperone complex with MCM2 and histone H3-H4 heterodimers (PMID: 33857403). DNAJC9 also plays a role as co-chaperone of the HSP70 family of molecular chaperone proteins. (PMID: 17182002, PMID: 33857403). DNAJC9 exhibits activity to assemble histones onto DNA (PMID: 33857403). DNAJC9 is an essential protein in many cancer cell types and the levels of the protein correlate with the rates at which cancer cells proliferate.

实验方案

Product Specific Protocols
WB protocol for DNAJC9 antibody 25444-1-APDownload protocol
IHC protocol for DNAJC9 antibody 25444-1-APDownload protocol
IF protocol for DNAJC9 antibody 25444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology.

Authors - Hexiao Shen
View Article
humanWB

bioRxiv

Mechanism of ASF1 Inhibition by CDAN1

Authors - Samantha F Sedor
View Article