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验证数据展示

  • WB analysis using 15809-1-AP

    WB analysis using 15809-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15809-1-AP (DNAL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 15809-1-AP

    IP experiment of HeLa using 15809-1-AP

    IP result of anti-DNAL1 (IP:15809-1-AP, 4ug; Detection:15809-1-AP 1:500) with HeLa cells lysate 2600 ug.

  • WB analysis of HeLa using 15809-1-AP

    WB analysis of HeLa using 15809-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15809-1-AP (DNAL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

DNAL1 Polyclonal antibody

DNAL1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 15809-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

C14orf168, DNAL1, Dynein light chain 1, axonemal

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15809-1-AP targets DNAL1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DNAL1 fusion protein Ag8545 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dynein, axonemal, light chain 1
别名 C14orf168, DNAL1, Dynein light chain 1, axonemal
计算分子量 151 aa, 17 kDa
观测分子量 22 kDa
GenBank蛋白编号BC005343
基因名称 DNAL1
Gene ID (NCBI) 83544
RRIDAB_2095365
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ4LDG9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAL1 was originally documented as the Chlamydomonas outer arm dynein axonemal light chain 1. DNAL1 has been documented to link components of the cytoskeleton with the molecular motor dynein, perhaps acting as a scaffold for larger functional structures (PMID: 22018492).

实验方案

Product Specific Protocols
WB protocol for DNAL1 antibody 15809-1-APDownload protocol
IP protocol for DNAL1 antibody 15809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols