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DNASE1L3 Polyclonal antibody

DNASE1L3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11330-1-AP

Print datasheet

Synonyms

Deoxyribonuclease gamma, deoxyribonuclease I like 3, DHP2, DNAS1L3, DNase gamma, DNase I homolog protein DHP2, DNase I like 3, DNASE1L3, Liver and spleen DNase, LS DNase, LSD

验证数据展示

  • WB analysis of Raji using 11330-1-AP

    WB analysis of Raji using 11330-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 11330-1-AP (DNASE1L3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 11330-1-AP

    WB analysis of HEK-293 using 11330-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11330-1-AP (DNASE1L3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, Raji cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11330-1-AP targets DNASE1L3 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen DNASE1L3 fusion protein Ag1873 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deoxyribonuclease I-like 3
Synonyms Deoxyribonuclease gamma, deoxyribonuclease I like 3, DHP2, DNAS1L3, DNase gamma, DNase I homolog protein DHP2, DNase I like 3, DNASE1L3, Liver and spleen DNase, LS DNase, LSD
Calculated Molecular Weight 305 aa, 36 kDa
Observed Molecular Weight 33-38 kDa
GenBank Accession NumberBC015831
Gene Symbol DNASE1L3
Gene ID (NCBI) 1776
RRIDAB_2095527
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13609
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Deoxyribonuclease 1-like 3 (DNASE1L3, DNase gamma, DNase Y, LS-DNase) is member of a DNASE1 protein family that is identified by similar biochemical properties such as Ca2+/Mg2+ dependency and an optimal pH of about 7.0 as well as by a high similarity in their nucleic acid and amino acid sequences [PMID:15796714]. It is an endonuclease and cleaves double-stranded DNA (and single-stranded DNA) producing 3'-OH/5'-P ends, which is Ca2+/Mg2+-dependent and inhibited by Zn2+, but not by monomeric actin [PMID:9665719]. In addition, it has been suggested to be involved in apoptotic DNA fragmentation of thymocytes neuronal PC12 cells, C2C12 myoblasts and of cell lines expressing the recombinant nuclease [PMID: 7957253, 9307016]

实验方案

Product Specific Protocols
WB protocol for DNASE1L3 antibody 11330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols