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验证数据展示

  • WB analysis of Raji using 11330-1-AP

    WB analysis of Raji using 11330-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 11330-1-AP (DNASE1L3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 11330-1-AP

    WB analysis of HEK-293 using 11330-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11330-1-AP (DNASE1L3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

DNASE1L3 Polyclonal antibody

DNASE1L3 Polyclonal Antibody for WB, ELISA
Cat No. 11330-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Deoxyribonuclease gamma, deoxyribonuclease I like 3, DHP2, DNAS1L3, DNase gamma, DNase I homolog protein DHP2, DNase I like 3, DNASE1L3, Liver and spleen DNase, LS DNase, LSD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Raji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11330-1-AP targets DNASE1L3 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 DNASE1L3 fusion protein Ag1873 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 deoxyribonuclease I-like 3
别名 Deoxyribonuclease gamma, deoxyribonuclease I like 3, DHP2, DNAS1L3, DNase gamma, DNase I homolog protein DHP2, DNase I like 3, DNASE1L3, Liver and spleen DNase, LS DNase, LSD
计算分子量 305 aa, 36 kDa
观测分子量 33-38 kDa
GenBank蛋白编号BC015831
基因名称 DNASE1L3
Gene ID (NCBI) 1776
RRIDAB_2095527
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13609
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Deoxyribonuclease 1-like 3 (DNASE1L3, DNase gamma, DNase Y, LS-DNase) is member of a DNASE1 protein family that is identified by similar biochemical properties such as Ca2+/Mg2+ dependency and an optimal pH of about 7.0 as well as by a high similarity in their nucleic acid and amino acid sequences [PMID:15796714]. It is an endonuclease and cleaves double-stranded DNA (and single-stranded DNA) producing 3'-OH/5'-P ends, which is Ca2+/Mg2+-dependent and inhibited by Zn2+, but not by monomeric actin [PMID:9665719]. In addition, it has been suggested to be involved in apoptotic DNA fragmentation of thymocytes neuronal PC12 cells, C2C12 myoblasts and of cell lines expressing the recombinant nuclease [PMID: 7957253, 9307016]

实验方案

Product Specific Protocols
WB protocol for DNASE1L3 antibody 11330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols