验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, LNCaP cells, MDA-MB-231 cells |
| Positive IP detected in | U-87 MG cells, HeLa cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15934-1-AP targets DNASE2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | DNASE2 fusion protein Ag8781 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | deoxyribonuclease II, lysosomal |
| Synonyms | DNASE2A, DNase II alpha, Deoxyribonuclease-2-alpha, Deoxyribonuclease II alpha, Deoxyribonuclease 2 alpha |
| Calculated Molecular Weight | 360 aa, 40 kDa |
| Observed Molecular Weight | 32-40 kDa |
| GenBank Accession Number | BC010419 |
| Gene Symbol | DNASE2 |
| Gene ID (NCBI) | 1777 |
| RRID | AB_2095546 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00115 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNASE2, or 'acid DNase,' hydrolyzes DNA under acidic conditions and is one of 2 distinct mammalian DNases. It hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. It has a key role in the degradation of nuclear DNA in cellular apoptosis during development [PMID:18812394]. Besieds, it is necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. It has been demonstrated to be responsible forDNA degradation in apoptotic cells engulfed by macrophages and exogenous DNA on the skin surface [PMID:23019102]. DNASE2 exists some isoforms with MV 39 and 34 kDa. (PMID: 14644493)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DNASE2 antibody 15934-1-AP | Download protocol |
| IHC protocol for DNASE2 antibody 15934-1-AP | Download protocol |
| IF protocol for DNASE2 antibody 15934-1-AP | Download protocol |
| IP protocol for DNASE2 antibody 15934-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Angelica sinensis polysaccharides ameliorate 5-FU-induced stress anemia via promoting extramedullary erythroblastic island central macrophage-mediated erythroid differentiation | ||
J Biol Chem Autolysosomal degradation of cytosolic chromatin fragments antagonizes oxidative stress-induced senescence. | ||
Pharmacol Res Oroxylin A regulates cGAS DNA hypermethylation induced by methionine metabolism to promote HSC senescence | ||
Mol Nutr Food Res Mangiferin protects DNase 2 abundance via Nrf2 activation to prevent cytosolic mtDNA accumulation during liver injury | ||
Transl Neurodegener Neuronal double-stranded DNA accumulation induced by DNase II deficiency drives tau phosphorylation and neurodegeneration |
