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- KD/KO Validated
DNASE2 Polyclonal antibody
DNASE2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, LNCaP cells, Jurkat cells, U-87 MG cells, MDA-MB-231 cells |
Positive IP detected in | U-87 MG cells, HeLa cells |
Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15934-1-AP targets DNASE2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | DNASE2 fusion protein Ag8781 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | deoxyribonuclease II, lysosomal |
Synonyms | DNASE2A, DNase II alpha, Deoxyribonuclease-2-alpha, Deoxyribonuclease II alpha, Deoxyribonuclease 2 alpha |
Calculated Molecular Weight | 360 aa, 40 kDa |
Observed Molecular Weight | 32-40 kDa |
GenBank Accession Number | BC010419 |
Gene Symbol | DNASE2 |
Gene ID (NCBI) | 1777 |
RRID | AB_2095546 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O00115 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for DNASE2 antibody 15934-1-AP | Download protocol |
IHC protocol for DNASE2 antibody 15934-1-AP | Download protocol |
IF protocol for DNASE2 antibody 15934-1-AP | Download protocol |
IP protocol for DNASE2 antibody 15934-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Int Immunopharmacol Angelica sinensis polysaccharides ameliorate 5-FU-induced stress anemia via promoting extramedullary erythroblastic island central macrophage-mediated erythroid differentiation | ||
J Biol Chem Autolysosomal degradation of cytosolic chromatin fragments antagonizes oxidative stress-induced senescence. | ||
Pharmacol Res Oroxylin A regulates cGAS DNA hypermethylation induced by methionine metabolism to promote HSC senescence | ||
Mol Nutr Food Res Mangiferin protects DNase 2 abundance via Nrf2 activation to prevent cytosolic mtDNA accumulation during liver injury | ||
Transl Neurodegener Neuronal double-stranded DNA accumulation induced by DNase II deficiency drives tau phosphorylation and neurodegeneration |