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DNASE2B Polyclonal antibody

DNASE2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22652-1-AP

Print datasheet

Synonyms

DNase II like acid DNase, DNase II beta, DLAD, Deoxyribonuclease-2-beta, Deoxyribonuclease II beta

验证数据展示

  • WB analysis of Jurkat using 22652-1-AP

    WB analysis of Jurkat using 22652-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22652-1-AP (DNASE2B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 22652-1-AP

    IF Staining of A549 using 22652-1-AP

    Immunofluorescent analysis of A549 cells using 22652-1-AP (DNASE2B antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IHC staining of human tonsillitis using 22652-1-AP

    IHC staining of human tonsillitis using 22652-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22652-1-AP (DNASE2B Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inJurkat cells, A549 cells
Positive IHC detected inhuman tonsillitis tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22652-1-AP targets DNASE2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen DNASE2B fusion protein Ag18246 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deoxyribonuclease II beta
Synonyms DNase II like acid DNase, DNase II beta, DLAD, Deoxyribonuclease-2-beta, Deoxyribonuclease II beta
Calculated Molecular Weight 361 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC126136
Gene Symbol DNASE2B
Gene ID (NCBI) 58511
RRIDAB_2879144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8WZ79
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DNASE2B antibody 22652-1-APDownload protocol
IHC protocol for DNASE2B antibody 22652-1-APDownload protocol
IF protocol for DNASE2B antibody 22652-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Int J Biochem Cell Biol

Heat shock factor 4 regulates lens epithelial cell homeostasis by working with lysosome and anti-apoptosis pathways.

Authors - Xiukun Cui
View Article