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验证数据展示

  • WB analysis of Jurkat using 22652-1-AP

    WB analysis of Jurkat using 22652-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22652-1-AP (DNASE2B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 22652-1-AP

    IF Staining of A549 using 22652-1-AP

    Immunofluorescent analysis of A549 cells using 22652-1-AP (DNASE2B antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IHC staining of human tonsillitis using 22652-1-AP

    IHC staining of human tonsillitis using 22652-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22652-1-AP (DNASE2B Antibody) at dilution of 1:50 (under 40x lens).

DNASE2B Polyclonal antibody

DNASE2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22652-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

DNase II like acid DNase, DNase II beta, DLAD, Deoxyribonuclease-2-beta, Deoxyribonuclease II beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, A549 cells
Positive IHC detected inhuman tonsillitis tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22652-1-AP targets DNASE2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 DNASE2B fusion protein Ag18246 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 deoxyribonuclease II beta
别名 DNase II like acid DNase, DNase II beta, DLAD, Deoxyribonuclease-2-beta, Deoxyribonuclease II beta
计算分子量 361 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC126136
基因名称 DNASE2B
Gene ID (NCBI) 58511
RRIDAB_2879144
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ8WZ79
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DNASE2B antibody 22652-1-APDownload protocol
IHC protocol for DNASE2B antibody 22652-1-APDownload protocol
IF protocol for DNASE2B antibody 22652-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Int J Biochem Cell Biol

Heat shock factor 4 regulates lens epithelial cell homeostasis by working with lysosome and anti-apoptosis pathways.

Authors - Xiukun Cui
View Article