• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 24362-1-AP

    WB analysis using 24362-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24362-1-AP (DNER antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 24362-1-AP

    IHC staining of mouse cerebellum using 24362-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24362-1-AP (DNER antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 24362-1-AP

    IHC staining of mouse cerebellum using 24362-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24362-1-AP (DNER antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

DNER Polyclonal antibody

DNER Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24362-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, pig

应用

WB, IHC, ELISA

别名

UNQ262/PRO299, Delta and Notch-like epidermal growth factor-related receptor, BET

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse cerebellum tissue, pig cerebellum tissue
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24362-1-AP targets DNER in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, pig
文献引用反应性human
免疫原 DNER fusion protein Ag19955 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 delta/notch-like EGF repeat containing
别名 UNQ262/PRO299, Delta and Notch-like epidermal growth factor-related receptor, BET
计算分子量 78 kDa
观测分子量110-130 kDa
GenBank蛋白编号BC035009
基因名称 DNER
Gene ID (NCBI) 92737
RRIDAB_3085732
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NFT8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Delta and Notch-like epidermal growth factor-related receptor (DNER) is a type I transmembrane protein that is specifically expressed in the developing and mature central nervous system (PMID: 11950833). It contains ten extracellular EGF-like repeats that are closely related to those of the developmentally important receptor Notch and its ligand Delta. DNER functions as a Notch ligand and mediates signaling through neuron-glia interactions (PMID: 15965470).

实验方案

Product Specific Protocols
WB protocol for DNER antibody 24362-1-APDownload protocol
IHC protocol for DNER antibody 24362-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Transcriptomics-Based Liquid Biopsy for Early Detection of Recurrence in Locally Advanced Gastric Cancer

Authors - Ping'an Ding
  • KD Validated
View Article