验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, fetal human brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human gliomas tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14737-1-AP targets DNM3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | DNM3 fusion protein Ag6381 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dynamin 3 |
| 别名 | T-dynamin, T dynamin, KIAA0820, EC:3.6.5.5, Dynamin-3 |
| 计算分子量 | 98 kDa |
| 观测分子量 | 97-100 kDa |
| GenBank蛋白编号 | BC064546 |
| 基因名称 | DNM3 |
| Gene ID (NCBI) | 26052 |
| RRID | AB_2093691 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UQ16 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNM3 (dynamin 3) is a member of dynamin proteins that participate in membrane rearrangements such as vesicular trafficking and phaocytosis. Three dynamin proteins encoded by different genes are present in mammals: DNM1 is mainly expressed in central nervous system, DNM2 ubiquitously expressed, and DNM3 expressed chiefly in the testis and brain. Western blot using this antibody specifically detected the 100 kDa DNM3 in mouse brain. It doesn't cross-react with other DNMs. (22977254, 21671749)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DNM3 antibody 14737-1-AP | Download protocol |
| IHC protocol for DNM3 antibody 14737-1-AP | Download protocol |
| IF protocol for DNM3 antibody 14737-1-AP | Download protocol |
| IP protocol for DNM3 antibody 14737-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics Modification of Crocodile Spermatozoa Refutes the Tenet That Post-testicular Sperm Maturation Is Restricted To Mammals | ||
Mol Cell Proteomics Proteomic profiling of crocodile spermatozoa refutes the tenet that post-testicular maturation is restricted to mammals. | ||
J Biol Chem Dynamin regulates specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa. | ||
Mol Hum Reprod Characterization of a novel role for the dynamin mechanoenzymes in the regulation of human sperm acrosomal exocytosis. | ||
Clin Exp Metastasis Identification and validation of genes with expression patterns inverse to multiple metastasis suppressor genes in breast cancer cell lines.
| ||
Biol Reprod Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis. |
