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DNMT1 Polyclonal antibody

DNMT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28754-1-AP

Print datasheet

Synonyms

EC:2.1.1.37, DNMT, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, CXXC9

验证数据展示

  • WB analysis using 28754-1-AP

    WB analysis using 28754-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28754-1-AP (DNMT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293T cells, HepG2 cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28754-1-AP targets DNMT1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen DNMT1 fusion protein Ag29479 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DNA (cytosine-5-)-methyltransferase 1
Synonyms EC:2.1.1.37, DNMT, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, CXXC9
Calculated Molecular Weight 1632 aa, 185 kDa
Observed Molecular Weight145 kDa and 184 kDa
GenBank Accession NumberBC126227
Gene Symbol DNMT1
Gene ID (NCBI) 1786
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26358
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with the molecular mass of 184 kDa and 145 kDa. (PMID: 10753866)

实验方案

Product Specific Protocols
WB protocol for DNMT1 antibody 28754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols