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DNMT1 Recombinant antibody, PBS Only (Detector)

DNMT1 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

rat, human

Applications

Cytometric bead array, Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

240302E2

Cat no : 82678-2-PBS

Print datasheet

Synonyms

DNMT, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, CXXC9, AIM

验证数据展示

  • WB analysis using 82678-2-RR (same clone as 82678-2-PBS)

    WB analysis using 82678-2-RR (same clone as 82678-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82678-2-RR (DNMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82678-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82678-2-RR (same clone as 82678-2-PBS)

    FC experiment of HeLa using 82678-2-RR (same clone as 82678-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DNMT1 Recombinant antibody (82678-2-RR, Clone:240302E2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82678-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00377-1

    Cytometric bead array standard curve of MP00377-1

    Cytometric bead array standard curve of MP00377-1, DNMT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82678-3-PBS. Detection antibody: 82678-2-PBS. Standard: Ag29479. Range: 1.56-100 ng/mL.

产品信息

82678-2-PBS targets DNMT1 as part of a matched antibody pair:

MP00377-1: 82678-3-PBS capture and 82678-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Indirect ELISA, WB Application Description
Tested Reactivity rat, human
Immunogen DNMT1 fusion protein Ag29479 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DNA (cytosine-5-)-methyltransferase 1
Synonyms DNMT, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, CXXC9, AIM
Calculated Molecular Weight 1632 aa, 185 kDa
GenBank Accession NumberBC126227
Gene Symbol DNMT1
Gene ID (NCBI) 1786
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26358
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C