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  • Featured Product
  • KD/KO Validated

DNMT3A Polyclonal antibody, PBS Only

DNMT3A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20954-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 20954-1-AP (same clone as 20954-1-PBS)

    WB analysis using 20954-1-AP (same clone as 20954-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20954-1-AP (DNMT3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • IF Staining of K-562 using 20954-1-AP (same clone as 20954-1-PBS)

    IF Staining of K-562 using 20954-1-AP (same clone as 20954-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using DNMT3A antibody (20954-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 20954-1-AP (same clone as 20954-1-PBS)

    WB analysis of HepG2 using 20954-1-AP (same clone as 20954-1-PBS)

    WB result of DNMT3A antibody (20954-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNMT3A transfected HepG2 cells. This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 20954-1-AP (same clone as 20954-1-PBS)

    IHC staining of human stomach cancer using 20954-1-AP (same clone as 20954-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNMT3A antibody included
  • IP experiment of HeLa using 20954-1-AP (same clone as 20954-1-PBS)

    IP experiment of HeLa using 20954-1-AP (same clone as 20954-1-PBS)

    IP result of anti-DNMT3A (IP:20954-1-AP, 4ug; Detection:20954-1-AP 1:5000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 20954-1-AP (same clone as 20954-1-PBS)

    IF Staining of HeLa using 20954-1-AP (same clone as 20954-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNMT3A antibody (20954-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy DNMT3A Ready-To-Use IHC Kit. DNMT3A primary antibody included.

产品信息

20954-1-PBS targets DNMT3A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DNMT3A fusion protein Ag15111 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DNA (cytosine-5-)-methyltransferase 3 alpha
Synonyms
Calculated Molecular Weight 912 aa, 102 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC043617
Gene Symbol DNMT3A
Gene ID (NCBI) 1788
RRIDAB_10733339
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6K1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a. It is expected to detect the 120-130 kDa DNMT3a but not 72-100 kDa DNMT3a2.