• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

DNPEP Polyclonal antibody

DNPEP Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15016-1-AP

Print datasheet

Synonyms

EC:3.4.11.21, DAP, ASPEP

验证数据展示

  • WB analysis using 15016-1-AP

    WB analysis using 15016-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15016-1-AP (DNPEP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 15016-1-AP

    WB analysis of MCF-7 using 15016-1-AP

    WB result of DNPEP antibody (15016-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNPEP transfected MCF-7 cells.

  • IP experiment of HEK-293 using 15016-1-AP

    IP experiment of HEK-293 using 15016-1-AP

    IP result of anti-DNPEP (IP:15016-1-AP, 3ug; Detection:15016-1-AP 1:500) with HEK-293 cells lysate 1760ug.

  • IF Staining of MCF-7 using 15016-1-AP

    IF Staining of MCF-7 using 15016-1-AP

    Immunofluorescent analysis of MCF-7 cells using 15016-1-AP (DNPEP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15016-1-AP targets DNPEP in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen DNPEP fusion protein Ag6966 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aspartyl aminopeptidase
Synonyms EC:3.4.11.21, DAP, ASPEP
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC000653
Gene Symbol DNPEP
Gene ID (NCBI) 23549
RRIDAB_2094293
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULA0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNPEP, also named as ASPEP and DAP, plays an important role in intracellular protein and peptide metabolism. It catalyzes the hydrolysis of N-terminal aspartyl and glutamyl residues.

实验方案

Product Specific Protocols
WB protocol for DNPEP antibody 15016-1-APDownload protocol
IF protocol for DNPEP antibody 15016-1-APDownload protocol
IP protocol for DNPEP antibody 15016-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell Biol

Chondrocyte-specific microRNA-140 regulates endochondral bone development and targets Dnpep to modulate bone morphogenetic protein signaling.

Authors - Nakamura Yukio Y
View Article