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验证数据展示

  • IHC staining of human lymphoma using 17382-1-AP

    IHC staining of human lymphoma using 17382-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 17382-1-AP (DNTT,TDT antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lymphoma using 17382-1-AP

    IHC staining of human lymphoma using 17382-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 17382-1-AP (DNTT,TDT antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of Raji using 17382-1-AP

    IP experiment of Raji using 17382-1-AP

    IP result of anti-DNTT,TDT (IP:17382-1-AP, 4ug; Detection:17382-1-AP 1:300) with Raji cells lysate 2000ug.

  • FC experiment of Jurkat using 17382-1-AP

    FC experiment of Jurkat using 17382-1-AP

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug DNTT,TDT Polyclonal antibody (17382-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

DNTT,TDT Polyclonal antibody

DNTT,TDT Polyclonal Antibody for IHC, FC (Intra), IP, ELISA
Cat No. 17382-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC (Intra), IP, ELISA

别名

DNTT, Terminal Deoxynucleotidyl Transferase, TDT, EC:2.7.7.31, DNA nucleotidylexotransferase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inRaji cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inJurkat cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17382-1-AP targets DNTT,TDT in IHC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原 DNTT,TDT fusion protein Ag11235 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 deoxynucleotidyltransferase, terminal
别名 DNTT, Terminal Deoxynucleotidyl Transferase, TDT, EC:2.7.7.31, DNA nucleotidylexotransferase
计算分子量 509 aa, 58 kDa
GenBank蛋白编号BC012920
基因名称 DNTT
Gene ID (NCBI) 1791
RRIDAB_2878399
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04053
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for DNTT,TDT antibody 17382-1-APDownload protocol
IP protocol for DNTT,TDT antibody 17382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols