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DNTT,TDT Polyclonal antibody

DNTT,TDT Polyclonal Antibody for IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 17382-1-AP

Print datasheet

Synonyms

DNTT, TDT, EC:2.7.7.31, DNA nucleotidylexotransferase

验证数据展示

  • IHC staining of human lymphoma using 17382-1-AP

    IHC staining of human lymphoma using 17382-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 17382-1-AP (DNTT,TDT antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lymphoma using 17382-1-AP

    IHC staining of human lymphoma using 17382-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 17382-1-AP (DNTT,TDT antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of Raji using 17382-1-AP

    IP experiment of Raji using 17382-1-AP

    IP result of anti-DNTT,TDT (IP:17382-1-AP, 4ug; Detection:17382-1-AP 1:300) with Raji cells lysate 2000ug.

  • FC experiment of Jurkat using 17382-1-AP

    FC experiment of Jurkat using 17382-1-AP

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug DNTT,TDT Polyclonal antibody (17382-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IP detected inRaji cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inJurkat cells

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17382-1-AP targets DNTT,TDT in IHC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, FC (Intra), IP, ELISA Application Description
Tested Reactivity human
Immunogen DNTT,TDT fusion protein Ag11235 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deoxynucleotidyltransferase, terminal
Synonyms DNTT, TDT, EC:2.7.7.31, DNA nucleotidylexotransferase
Calculated Molecular Weight 509 aa, 58 kDa
GenBank Accession NumberBC012920
Gene Symbol DNTT
Gene ID (NCBI) 1791
RRIDAB_2878399
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04053
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for DNTT,TDT antibody 17382-1-APDownload protocol
IP protocol for DNTT,TDT antibody 17382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols