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验证数据展示

  • WB analysis using 20575-1-AP

    WB analysis using 20575-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20575-1-AP (DOC2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 20575-1-AP

    IHC staining of mouse brain using 20575-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20575-1-AP (DOC2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 20575-1-AP

    IHC staining of mouse brain using 20575-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20575-1-AP (DOC2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DOC2A Polyclonal antibody

DOC2A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20575-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Doc2, Doc2 alpha, DOC2A, double C2 like domains, alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse testis tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20575-1-AP targets DOC2A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 double C2-like domains, alpha
别名 Doc2, Doc2 alpha, DOC2A, double C2 like domains, alpha
计算分子量 44 kDa
观测分子量18-44 kDa
GenBank蛋白编号NM_003586
基因名称 DOC2A
Gene ID (NCBI) 8448
RRIDAB_3085608
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14183
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DOC2, Double C2-like domain-containing protein alpha, is involved in Ca(2+)-dependent neurotransmitter release. DOC2A and DOC2B are sensors for neuronal activity with unique calcium-dependent and kinetic properties (PMID: 16515538). DOC2A is mainly expressed in brain and also expressed in testis (PMID: 7826360).

实验方案

Product Specific Protocols
WB protocol for DOC2A antibody 20575-1-APDownload protocol
IHC protocol for DOC2A antibody 20575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols