验证数据展示
经过测试的应用
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16834-1-AP targets DOCK10 in IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | DOCK10 fusion protein Ag10486 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dedicator of cytokinesis 10 |
| 别名 | Zizimin-3, Zizimin 3, ZIZ3, Nbla10300, DRIP2 |
| 计算分子量 | 542 aa, 62 kDa |
| 观测分子量 | 250 kDa |
| GenBank蛋白编号 | BC015018 |
| 基因名称 | DOCK10 |
| Gene ID (NCBI) | 55619 |
| RRID | AB_2878323 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96BY6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DOCK10 (Dedicator of Cytokinesis 10) is a protein-coding gene that belongs to the DOCK family of guanine nucleotide exchange factors (GEFs). It is involved in regulating the activity of Rho GTPases, particularly Rac1 and Cdc42, which play crucial roles in various cellular processes.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for DOCK10 antibody 16834-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |