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  • Featured Product
  • KD/KO Validated

DOCK2 Monoclonal antibody, PBS Only

DOCK2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D7A8

Cat no : 66969-1-PBS

Print datasheet

Synonyms

dedicator of cytokinesis 2, DOCK2, FLJ46592, KIAA0209

验证数据展示

  • WB analysis of Jurkat using 66969-1-Ig (same clone as 66969-1-PBS)

    WB analysis of Jurkat using 66969-1-Ig (same clone as 66969-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66969-1-Ig (same clone as 66969-1-PBS)

    IHC staining of human liver cancer using 66969-1-Ig (same clone as 66969-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66969-1-Ig (DOCK2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66969-1-Ig (same clone as 66969-1-PBS)

    IHC staining of human liver cancer using 66969-1-Ig (same clone as 66969-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66969-1-Ig (DOCK2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66969-1-Ig (same clone as 66969-1-PBS)

    IF Staining of HeLa using 66969-1-Ig (same clone as 66969-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DOCK2 antibody (66969-1-Ig, Clone: 1D7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

产品信息

66969-1-PBS targets DOCK2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen DOCK2 fusion protein Ag28582 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dedicator of cytokinesis 2
Synonyms dedicator of cytokinesis 2, DOCK2, FLJ46592, KIAA0209
Calculated Molecular Weight 212 kDa, 38 kDa
Observed Molecular Weight 200-210 kDa
GenBank Accession NumberBC016996
Gene Symbol DOCK2
Gene ID (NCBI) 1794
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ92608
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DOCK2, also named as KIAA0209, belongs to the DOCK family. It is involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. DOCK2 activates RAC1 and RAC2 small GTPases, probably by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. DOCK2 may also participate in IL2 transcriptional activation via the activation of RAC2. The antibody is specific to DOCK2.