DOCK4 Polyclonal antibody

DOCK4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 21861-1-AP

Print datasheet

Synonyms

KIAA0716, dedicator of cytokinesis 4



经过测试的应用

Positive WB detected inHEK-293 cells, HEK-293T cells
Positive IP detected inHeLa cells, HEK-293T cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue, human prostate cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:150-1:600
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21861-1-AP targets DOCK4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen DOCK4 fusion protein Ag16516 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dedicator of cytokinesis 4
Synonyms KIAA0716, dedicator of cytokinesis 4
Calculated Molecular Weight 2011 aa, 230 kDa
Observed Molecular Weight 225 kDa
GenBank Accession NumberBC117689
Gene Symbol DOCK4
Gene ID (NCBI) 9732
RRIDAB_2878930
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N1I0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DOCK4, originally identified as a product of a gene which is deleted during tumor progression, is a member of DOCK180 family proteins. Dock4 has been found recently to be associated with several neuropsychiatric diseases, including autism, dyslexia, and schizophrenia. Multiple studies in fibroblasts then confirmed that Dock4 is capable of controlling cell migration by transducing several upstream signals, such as Wnt, platelet-derived growth factor, and RhoG, toward activation of Rac1. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers.

实验方案

Product Specific Protocols
WB protocol for DOCK4 antibody 21861-1-APDownload protocol
IHC protocol for DOCK4 antibody 21861-1-APDownload protocol
IF protocol for DOCK4 antibody 21861-1-APDownload protocol
IP protocol for DOCK4 antibody 21861-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Proc Natl Acad Sci U S A

Up-regulated cytotrophoblast DOCK4 contributes to over-invasion in placenta accreta spectrum.

Authors - Leah McNally
humanWB, IHC

Front Cell Dev Biol

USP36-Mediated Deubiquitination of DOCK4 Contributes to the Diabetic Renal Tubular Epithelial Cell Injury via Wnt/β-Catenin Signaling Pathway.

Authors - Suwei Zhu
humanWB

Front Oncol

miR-33b-3p Acts as a Tumor Suppressor by Targeting DOCK4 in Prostate Cancer.

Authors - Yu Mei
humanWB

Science

Multiscale photocatalytic proximity labeling reveals cell surface neighbors on and between cells

Authors - Zhi Lin