DOCK4 Polyclonal antibody
DOCK4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HEK-293T cells |
Positive IP detected in | HeLa cells, HEK-293T cells |
Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue, human prostate cancer tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:150-1:600 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21861-1-AP targets DOCK4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | DOCK4 fusion protein Ag16516 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | dedicator of cytokinesis 4 |
Synonyms | KIAA0716, dedicator of cytokinesis 4 |
Calculated Molecular Weight | 2011 aa, 230 kDa |
Observed Molecular Weight | 225 kDa |
GenBank Accession Number | BC117689 |
Gene Symbol | DOCK4 |
Gene ID (NCBI) | 9732 |
RRID | AB_2878930 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8N1I0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for DOCK4 antibody 21861-1-AP | Download protocol |
IHC protocol for DOCK4 antibody 21861-1-AP | Download protocol |
IF protocol for DOCK4 antibody 21861-1-AP | Download protocol |
IP protocol for DOCK4 antibody 21861-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Proc Natl Acad Sci U S A Up-regulated cytotrophoblast DOCK4 contributes to over-invasion in placenta accreta spectrum. | ||
Front Cell Dev Biol USP36-Mediated Deubiquitination of DOCK4 Contributes to the Diabetic Renal Tubular Epithelial Cell Injury via Wnt/β-Catenin Signaling Pathway. | ||
Science Multiscale photocatalytic proximity labeling reveals cell surface neighbors on and between cells |