• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 29938-1-AP

    WB analysis using 29938-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 29938-1-AP

    WB analysis of A549 using 29938-1-AP

    WB result of DOCK5 antibody (29938-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-DOCK5 transfected A549 cells.

  • IP experiment of DU 145 using 29938-1-AP

    IP experiment of DU 145 using 29938-1-AP

    IP result of anti-DOCK5 (IP:29938-1-AP, 4ug; Detection:29938-1-AP 1:2000) with DU 145 cells lysate 1280 ug.

  • IHC staining of mouse kidney using 29938-1-AP

    IHC staining of mouse kidney using 29938-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29938-1-AP (DOCK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 29938-1-AP

    IF Staining of U2OS using 29938-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using DOCK5 antibody (29938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

DOCK5 Polyclonal antibody

DOCK5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 29938-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

dedicator of cytokinesis 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inLNCaP cells, Jurkat cells, A549 cells, DU 145 cells, NIH3T3 cells, rat brain tissue, NIH/3T3 cells, mouse brain tissue
Positive IP detected inDU 145 cells
Positive IHC detected inmouse kidney tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29938-1-AP targets DOCK5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DOCK5 fusion protein Ag32193 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dedicator of cytokinesis 5
别名 dedicator of cytokinesis 5
计算分子量 1870 aa, 215 kDa
观测分子量190 kDa
GenBank蛋白编号BC137175
基因名称 DOCK5
Gene ID (NCBI) 80005
RRIDAB_3086194
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H7D0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DOCK5 is a member of the dedicator of cytokinesis protein family. Members of this family act as guanine nucleotide exchange factors for small Rho family G proteins. DOCK5 is thought to associate with adaptors CRK and CRKL, and function in regulation of intestinal epithelial cell spreading and migration on collagen IV.

实验方案

Product Specific Protocols
WB protocol for DOCK5 antibody 29938-1-APDownload protocol
IHC protocol for DOCK5 antibody 29938-1-APDownload protocol
IF protocol for DOCK5 antibody 29938-1-APDownload protocol
IP protocol for DOCK5 antibody 29938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols