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DOCK6 Polyclonal antibody

DOCK6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25087-1-AP

Print datasheet

Synonyms

dedicator of cytokinesis 6, DOCK6, KIAA1395, ZIR1

验证数据展示

  • WB analysis using 25087-1-AP

    WB analysis using 25087-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 25087-1-AP (DOCK6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 25087-1-AP

    IHC staining of human colon using 25087-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25087-1-AP (DOCK6 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 25087-1-AP

    IHC staining of human colon using 25087-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25087-1-AP (DOCK6 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25087-1-AP is DOCK6 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen DOCK6 fusion protein Ag18901 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dedicator of cytokinesis 6
Synonyms dedicator of cytokinesis 6, DOCK6, KIAA1395, ZIR1
Calculated Molecular Weight 2047 aa, 230 kDa
Observed Molecular Weight 230 kDa
GenBank Accession NumberBC146786
Gene Symbol DOCK6
Gene ID (NCBI) 57572
RRIDAB_2879889
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96HP0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DOCK6 antibody 25087-1-APDownload protocol
IHC protocol for DOCK6 antibody 25087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Cell Biol

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Authors - Li Wang
View Article
humanWB

BMC Med Genomics

Attenuated clinical and osteoclastic phenotypes of Paget's disease of bone linked to the p.Pro392Leu/SQSTM1 mutation by a rare variant in the DOCK6 gene.

Authors - Mariam Dessay
View Article