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验证数据展示

  • WB analysis using 25087-1-AP

    WB analysis using 25087-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 25087-1-AP (DOCK6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 25087-1-AP

    IHC staining of human colon using 25087-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25087-1-AP (DOCK6 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 25087-1-AP

    IHC staining of human colon using 25087-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25087-1-AP (DOCK6 Antibody) at dilution of 1:200 (under 40x lens).

DOCK6 Polyclonal antibody

DOCK6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25087-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA and More (1)

别名

dedicator of cytokinesis 6, DOCK6, KIAA1395, ZIR1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25087-1-AP targets DOCK6 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原 DOCK6 fusion protein Ag18901 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dedicator of cytokinesis 6
别名 dedicator of cytokinesis 6, DOCK6, KIAA1395, ZIR1
计算分子量 2047 aa, 230 kDa
观测分子量 230 kDa
GenBank蛋白编号BC146786
基因名称 DOCK6
Gene ID (NCBI) 57572
RRIDAB_2879889
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96HP0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DOCK6 antibody 25087-1-APDownload protocol
IHC protocol for DOCK6 antibody 25087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Cell Biol

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Authors - Li Wang
View Article
humanWB

BMC Med Genomics

Attenuated clinical and osteoclastic phenotypes of Paget's disease of bone linked to the p.Pro392Leu/SQSTM1 mutation by a rare variant in the DOCK6 gene.

Authors - Mariam Dessay
View Article