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DOCK7 Monoclonal antibody

DOCK7 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A4A6

Cat no : 67842-1-Ig

Print datasheet

Synonyms

dedicator of cytokinesis 7, DOCK7, KIAA1771, ZIR2

验证数据展示

  • WB analysis using 67842-1-Ig

    WB analysis using 67842-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67842-1-Ig (DOCK7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, PC-12 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, NIH3T3 cells, 4T1 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67842-1-Ig targets DOCK7 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen DOCK7 fusion protein Ag28417 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name dedicator of cytokinesis 7
Synonyms dedicator of cytokinesis 7, DOCK7, KIAA1771, ZIR2
Calculated Molecular Weight 2109 aa, 239 kDa
Observed Molecular Weight243 kDa
GenBank Accession NumberBC016392
Gene Symbol DOCK7
Gene ID (NCBI) 85440
RRIDAB_2918604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96N67
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

DOCK 7 (dedicator of cytokinesis 7), also known as ZIR2, is a member of the DOCK180-related protein superfamily. Expressed mainly in neuronal cells, DOCK 7 is a guanine nucleotide exchange factor (GEF) for small GTPases, Rac1 and Cdc42, which are the major regulators of actin cytoskeleton. Multiple isoforms of DOCK 7 exist due to alternative splicing events.

实验方案

Product Specific Protocols
WB protocol for DOCK7 antibody 67842-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols