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DOHH Polyclonal antibody

DOHH Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18226-1-AP

Print datasheet

Synonyms

HLRC1, HEAT-like repeat-containing protein 1, hDOHH, EC:1.14.99.29

验证数据展示

  • WB analysis using 18226-1-AP

    WB analysis using 18226-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18226-1-AP (DOHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inDU 145 cells, LNCaP cells, U-87 MG cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18226-1-AP targets DOHH in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen DOHH fusion protein Ag13072 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deoxyhypusine hydroxylase/monooxygenase
Synonyms HLRC1, HEAT-like repeat-containing protein 1, hDOHH, EC:1.14.99.29
Calculated Molecular Weight 33 kDa
Observed Molecular Weight30-35 kDa
GenBank Accession NumberBC002817
Gene Symbol DOHH
Gene ID (NCBI) 83475
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BU89
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

eoxyhypusine hydroxylase (DOHH) is the enzyme catalyzing the second step in the post-translational synthesis of hypusine [Nε-(4-amino-2-hydroxybutyl)lysine] in the eukaryotic initiation factor 5A (eIF5A). DOHH has been reported to mediate several crucial cellular functions, including cellular proliferation, differentiation and apoptosis. Moreover, previous studies have established that DOHH is highly involved in several essential biological processes driving human diseases including cancer growth, malarial drug resistance, and HIV-1 replication. In particular, the DOHH/eIF5A signaling pathway was revealed to mediate the ability of nerve growth factor to enhance neuronal growth and survival, highlighting the importance of DOHH in neuroprotection(PMID: 35007708, PMID: 16371467, PMID: 22908221, PMID: 19706422).

实验方案

Product Specific Protocols
WB protocol for DOHH antibody 18226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols