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DOK1 Polyclonal antibody

DOK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17822-1-AP

Print datasheet

Synonyms

pp62, P62DOK, Docking protein 1

验证数据展示

  • WB analysis of K-562 using 17822-1-AP

    WB analysis of K-562 using 17822-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17822-1-AP (DOK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 17822-1-AP

    IHC staining of human ovary cancer using 17822-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17822-1-AP (DOK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17822-1-AP targets DOK1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen DOK1 fusion protein Ag12189 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name docking protein 1, 62kDa (downstream of tyrosine kinase 1)
Synonyms pp62, P62DOK, Docking protein 1
Calculated Molecular Weight 48 aa, 52 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC111055
Gene Symbol DOK1
Gene ID (NCBI) 1796
RRIDAB_2274420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99704
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The downstream of tyrosine kinase 1gene (DOK1) has emerged as a newly identified tumor suppressor gene that encodes a multi-domain adapter protein and acts as a negative regulator of signaling pathways involved in several cellular functions. DOK1 inhibits cell proliferation, down regulates MAP kinase activity, and has an opposing role in leukemogenesis and promotes cell spreading, motility, and apoptosis. (PMID: 24809689)

实验方案

Product Specific Protocols
WB protocol for DOK1 antibody 17822-1-APDownload protocol
IHC protocol for DOK1 antibody 17822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols