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DOK2 Polyclonal antibody

DOK2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14087-1-AP

Print datasheet

Synonyms

Docking protein 2, docking protein 2, 56kDa, DOK2, p56(dok 2), p56DOK, p56dok 2

验证数据展示

  • WB analysis of Jurkat using 14087-1-AP

    WB analysis of Jurkat using 14087-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14087-1-AP (DOK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 14087-1-AP

    IHC staining of human lymphoma using 14087-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 14087-1-AP (DOK2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lymphoma using 14087-1-AP

    IHC staining of human lymphoma using 14087-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 14087-1-AP (DOK2 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

14087-1-AP targets DOK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DOK2 fusion protein Ag5218 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name docking protein 2, 56kDa
Synonyms Docking protein 2, docking protein 2, 56kDa, DOK2, p56(dok 2), p56DOK, p56dok 2
Calculated Molecular Weight 412 aa, 45 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC032623
Gene Symbol DOK2
Gene ID (NCBI) 9046
RRIDAB_2230792
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60496
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Docking protein 2 (Dok2) is an adapter protein which is involved in hematopoiesis. Dok2 is able to control Klf1 expression by transcriptional regulation through directly binding to its promoter region (PMID: 25075100).

实验方案

Product Specific Protocols
WB protocol for DOK2 antibody 14087-1-APDownload protocol
IHC protocol for DOK2 antibody 14087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Histopathology

Expression of T-cell receptor signalling pathway components in extranodal NK/T-cell lymphoma.

Authors - Tomoko Miyata-Takata
View Article