DOK3 Polyclonal antibody
DOK3 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, Ramos cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
15749-1-AP targets DOK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | DOK3 fusion protein Ag8221 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | docking protein 3 |
| Synonyms | docking protein 3, DOK3, DOKL |
| Calculated Molecular Weight | 496aa,53 kDa; 330aa,36 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC004564 |
| Gene Symbol | DOK3 |
| Gene ID (NCBI) | 79930 |
| RRID | AB_2095099 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7L591 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The adaptor proteins downstream of kinase 1 (DOK1) to DOK3 are mainly found in immune cells, and they are closely related, with some functional redundancy, although each DOK protein has distinct properties and functions. DOK1 to DOK3 inhibit LPS responses. DOK proteins have an N-terminal pleckstrin homology (PH) domain, a phosphotyrosine-binding (PTB) domain, and four tyrosine-containing motifs that can bind to Src homology 2 (SH2) domains when phosphorylated. DOK3 was also shown to have an inhibitory role as it restricts Ca2+ and JNK activation in B cell receptor signaling. The MW of this protein is 53 kDa, and this antibody specially recognises the 53 kDa protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DOK3 antibody 15749-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Elevated expression of DOK3 indicates high suppressive immune cell infiltration and unfavorable prognosis of gliomas. |