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DOT1L Polyclonal antibody

DOT1L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28145-1-AP

Print datasheet

Synonyms

Lysine N methyltransferase 4, KMT4, KIAA1814, H3 K79 HMTase, DOT1 like protein

验证数据展示

  • WB analysis using 28145-1-AP

    WB analysis using 28145-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28145-1-AP (DOT1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inPC-12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28145-1-AP targets DOT1L in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, rat
Immunogen DOT1L fusion protein Ag27229 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DOT1-like, histone H3 methyltransferase (S. cerevisiae)
Synonyms Lysine N methyltransferase 4, KMT4, KIAA1814, H3 K79 HMTase, DOT1 like protein
Calculated Molecular Weight 185 kDa
Observed Molecular Weight185~200 kDa
GenBank Accession NumberNM_032482
Gene Symbol DOT1L
Gene ID (NCBI) 84444
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TEK3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DOT1L (Disruptor of telomeric silencing 1-like) is the only known histone methyltransferse catalyzing H3K79 methylation which is an activating mark for gene transcription by promoting transcriptional elongation (PMID: 34187895). DOT1L forms a complex with AF10/AF17 and ENL/AF9, is dysregulated in mixed-lineage leukemia (MLLr), and is believed to regulate transcriptional elongation without direct evidence. DOT1L has been reported to directly interact with RNAPII phosphorylated on Ser2 and/or Ser5 of the C-terminal domain (CTD) of its largest subunit. Loss of Dot1L results in yolk sac angiogenesis defects and embryonic lethality (PMID: 18787701).

实验方案

Product Specific Protocols
WB protocol for DOT1L antibody 28145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols